S U M M A R YWhen 3-0-methyl glucose is actively transported into the fungus Dendryphiella salina metabolizable soluble carbohydrate is converted into polysaccharide and other insoluble material such that the total soluble carbohydrate concentration remains constant. Uptake of 3-0-methyl glucose from 1-0 mM solution is inhibited by equimolar glucose, fructose, mannitol or arabitol and by potassium acetate, both ionic species being involved. The results are discussed in relation to the control of hyphal osmotic pressure. I N T R O D U C T I O NThe kinetics of glucose transport into cells are often best studied by using those analogues of glucose which are not metabolized. These analogues are used because uptake can be assayed radiochemically and active transport can be demonstrated unequivocally. Usuaiiy the amount of the analogue which has entered the cell is determined. If the analogue can be obtained with a high specific activity, the relationship between rate of uptake and the external concentration can be determined with a considerable degree of accuracy because the experimental period can be short enough to minimize changes of concentration in the medium. However, because the analogue is not metabolized, it is often assumed that its presence inside the cell is without effect on metabolism. This paper reports data for the marine hyphomycete Dendryphiella salina, demonstrating the incorrectness of this assumption.Detailed information about the carbohydrate metabolism of Dendryphiella salina has been provided already (Allaway & Jennings, I 970 b ; Holligan & Jennings, I 972 a, b, c, I 973). The major soluble carbohydrates are mannitol and arabitol, the relative amounts being determined by the form in which the nitrogen is supplied to the external medium.

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