Market adaptaram à eletroforese métodos histoquímicos de coloração, para localizar as zonas de atividade enzimática diretamente no meio suporte. Linn e Arber em 1968 descobriram as enzimas de restrição que cortam o DNA em pontos específicos fornecendo as respaldo para, em 1973, Stanley, Cohen e Brown isolarem genes e introduzi-los em outro organismo via Agrobacterium. A manipulação do material genético começou efetivamente em 1973, na Universidade de Stanford, nos EUA, quando o Dr. Paul Berg conseguiu unir sequência de DNA de Escherichia coli à do vírus Simian papilona. Esse feito pode ser considerado o marco zero da denominada 'era genômica' (RAMALHO & LAMBERT, 2004). Posteriormente, em fevereiro de 1977, os bioquímicos norte-americanos David Glover e David Hogness, da Stanford University School of Medicine, foram os primeiros a relatar, que os genes eucarióticos codificadores de RNAs ribossomais são interrompidos. No mesmo ano, vários pesquisadores, como Richard Roberts do Cold Spring Harbour Laboratory (EUA), Philip Sharp, do
Objective: to analyze the knowledge of students of the Undergraduate Nursing Course of a Private Higher Education Institution in Belem, State of Pará, Brazil on Basic Life Support. Method: A cross-sectional, descriptive, exploratory study with a quantitative approach was carried out from September to October 2018. Result: In the evaluation of knowledge about Basic Life Support, on a scale of 0 to 100 points, it was verified that the lowest grade was 20.6 points and the highest was 90.4. The mean was 63.5 points, with a standard deviation of 15.5. The median was 64 points, with the interquartile deviation of 20 points (54 to 74 points). It was evidenced that 62% of the students had a grade lower than 70 points. And 19% scored less than 50 points. The population studied has reduced, and sometimes inadequate, knowledge about cardiorespiratory arrest and cardiopulmonary resuscitation, which may compromise the care provided, leading to damages to resuscitation and, consequently, to contribute to the appearance and / or aggravation of sequels, impacting on increased morbidity and mortality. Conclusion: it is understood that it is fundamental to establish training and evaluation of these, in a theoretical and practical, as a way to optimize and consolidate knowledge even during academic training.
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