The fluorescent probe has become an important method for accurate detection of H 2 O 2 , with advantages of simple operation, high sensitivity, good selectivity, and real-time dynamic monitoring. This paper reviews the research progress in organic small-molecule fluorescent probe H 2 O 2 detection methods that are based on different recognition reactions. In addition, the application prospect of fluorescent probes in the detection of trace H 2 O 2 is anticipated.