Purpose:The purpose of this study was to investigate the relationship between anthropometric indices and lipid profile in-office employees.Methods: This descriptive study was performed in Tehran, Iran. In total 294 office employees (166 males and 83 females) participated as samples study in this research. Blood samples (TC, TG, HDL-C, LDL-C and LDL-C to HDL-C ratio) were taken from the brachial vein in sitting position and fasting state. Then anthropometric indices includingbody mass index (BMI), waist circumferences (WC) and waist-hip ratio (WHR) were measured and recorded. All statistical analyses were conducted with "SPSS 21". Results: There was a positive and significant correlation between TG and LDL-C/HDL-C ratio with BMI, WC and WHR. There was also a direct and significant correlation between WHR and LDL-C. On the other hand, there was an inverse correlation between HDL with BMI, WC and WHR. Moreover, anthropometric indices (BMI, WC and WHR) were significantly higher in the older group than the younger group. Also, triglycerides, LDL-C and also, BMI, WC and WHR were significantly higher in men than women; but, HDL-C was significantly higher in women. Conclusion: Regarding the association of anthropometric indices with lipid profile and its significant differences across age and gender groups, these parameters can be used to evaluate and screen cardiovascular and metabolic disease-related risk factors.