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Human beings use plants for a multitude of purposes, of which, a globally prominent one is for their medicinal values. Medicinal plants serve as a major source of income for high-altitude inhabitants in the Himalayas, particularly in countries like India, Nepal, and Bhutan. However, people here harvest huge volumes of medicinal plants indiscriminately, risking their sustainability and causing anthropogenic threats. The global market demands for Uttarakhand’s medicinal and aromatic plants are high, and several of these plants are exclusively found in Himalayan states. Himalayan states have a variety of agro-geo-climatic conditions that are ideal for medicinal and aromatic plants. Uttarakhand, being home to a diverse range of wildlife, as well as fragrant plants, has subtropical to tropical soils and agro-climatic conditions. The Alpine is a biodiversity hotspot with a diverse assortment of wild animals. But as a result of the low agricultural production, there is a lack of industrial growth leading to underdevelopment and poverty in such areas. Consequently, Himalayan inhabitants travel to the plains to take advantage of biodiversity to improve their lifestyle and socioeconomic status.

Rose bengal dye is extensively used in the textile and photochemical industries as well as its presence in aquatic environment through industrial effluents cause severe toxic effects on the human health, aquatic life and to the environment. Therefore, in the present study, an attempt has been made to remove the rose bengal dye where it adsorbed on the surface of the adsorbent i.e. ZnO nanoparticles synthesized by aqueous stem extract of Arisaema tortuosum and separated by centrifugal method. The synthesized nanoparticles were characterized by UV-Visible, XRD, FTIR SEM and TEM techniques that confirmed the formation of zinc oxide in the nanorange. Finally, change in concentration of rose bengal dye in the aqueous medium after removal by centrifugal method was estimated by quantitative analysis using UV-Vis spectrophotometer. The parameters studied were adsorbent dosage, adsorbate dosage and contact time. The results have shown that ZnO NPs were very efficient to remove the rose bengal dye pollutant from aqueous medium which may find huge application in the wastewater treatment.

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