Influence of aqueous leaf extracts, leaf residue, and leached-leaf residue of houndstongue, a noxious rangeland weed, on seedling emergence of forage grasses was studied. Ultraviolet-B (UV-B) effects during houndstongue growth on subsequent germination and growth-inhibitory activity of leaf extracts were investigated. Addition of glasshouse-grown houndstongue leaf extract to mineral soil decreased emergence of crested wheatgrass by 13% and prairie junegrass by 20% at 14 d after sowing. Idaho fescue emergence was unaffected. Incorporation of houndstongue leaf- and leached-leaf residue into soil (0.4 g residue : 20 g soil) delayed emergence of forage grasses. At 14 d after sowing, houndstongue leaf residue spread on the soil surface (0.2 g residue : 20 g soil) tended to inhibit seedling emergence more than leaf residues incorporated into soil. In separate experiments, houndstongue plants were grown at 0, 4, 7, and 11 kJ/m2/d biologically effective UV-B radiation for 6 wk, and leaf extracts (0.5, 1, 2, and 4% wt/v) were prepared. Exposure of houndstongue to increasing UV-B dose during plant growth generally increased the inhibitory activity of their leaf extract on prairie junegrass germination. Crested wheatgrass and Idaho fescue seedlings incubated in extracts of houndstongue leaves exposed to UV-B, compared with leaves grown in a UV-B–free environment, had decreased root lengths. Leaf extracts of plants exposed to elevated UV-B levels had higher absorbance at 300 nm, indicating greater concentration of UV-B–absorbing compounds. This study suggests houndstongue leaf extracts and residues inhibit seed germination and seedling emergence and that UV-B may enhance their allelopathic influence on some forage grasses. Field studies are needed to confirm the allelopathic influence of houndstongue under rangeland conditions.

Fusarium wilt, caused by Fusarium oxysporum, is one of the most notorious diseases of cash crops. The use of microbial fungicides is an effective measure for controlling Fusarium wilt, and the genus Bacillus is an important resource for the development of microbial fungicides. Fusaric acid (FA) produced by F. oxysporum can inhibit the growth of Bacillus, thus affecting the control efficacy of microbial fungicides. Therefore, screening FA-tolerant biocontrol Bacillus may help to improve the biocontrol effect on Fusarium wilt. In this study, a method for screening biocontrol agents against Fusarium wilt was established based on tolerance to FA and antagonism against F. oxysporum. Three promising biocontrol bacteria, named B31, F68, and 30833, were obtained to successfully control tomato, watermelon, and cucumber Fusarium wilt. Strains B31, F68, and 30833 were identified as B. velezensis by phylogenetic analysis of the 16S rDNA, gyrB, rpoB, and rpoC gene sequences. Coculture assays revealed that strains B31, F68, and 30833 showed increased tolerance to F. oxysporum and its metabolites compared with B. velezensis strain FZB42. Further experiments confirmed that 10 µg/mL FA completely inhibited the growth of strain FZB42, while strains B31, F68, and 30833 maintained normal growth at 20 µg/mL FA and partial growth at 40 µg/mL FA. Compared with strain FZB42, strains B31, F68, and 30833 exhibited significantly greater tolerance to FA.