Rabbit antibody IgG was bound onto aminoalkylsilyl or plain glass rods by simple adsorption. For comparison, rabbit antibody IgG was also bound onto glutaraldehyde-activated aminoalkylsilyl glass rods. These antibody-glass rods were tested by the sandwich procedure using Fab' fragments of rabbit antibody conjugated with beta-D-galactosidase from Escherichia coli. The glutaraldehyde-activated aminoalkylsilyl glass showed the largest capacity to bind antigen and the plain glass showed the smallest. However, the antibody-glass rods prepared by simple adsorption were as useful for the sandwich immunoassay of macromolecular antigens as those prepared with glutaraldehyde. With all the antibody-glass rods prepared, 0.1 to 10 fmol of ornithine delta-aminotransferase from rat liver and 2,4-dinitrophenyl human IgG were measurable. More than 10 fmol of the antigens may be measurable with larger amounts of the antibody-beta-D-galactosidase complexes, although the non-specific binding of the complexes to the solid phase increases to limit the sensitivity of the immunoassay.

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Objective Self‐focused attention is a maintaining factor for social anxiety disorder. It was shown that self‐focused attention correlates with trait mindfulness, but not with attention control. This study examined the reliability and validity of the Japanese version of the self‐focused attention (J‐SFA) scale. Method Students (N = 502) completed a set of questionnaires, measuring self‐focused attention, social anxiety symptoms, trait mindfulness, and attention control. Results A confirmatory factor analysis indicated that the J‐SFA scale has a bi‐factor structure. The Cronbach's α coefficient was high. Correlation analysis showed that each factor of the J‐SFA scale was significantly weakly to moderately correlated with social anxiety symptoms and trait mindfulness, and the J‐SFA scale's factors were significantly or non‐significantly very weakly correlated with attentional control functions. Conclusion The findings indicate that the J‐SFA demonstrated satisfactory reliability and validity for our sample and provide impetus for future research into the measure for clinical and nonclinical samples in Japan.