Work life balance has been the subject of much discussion all over the world among people of all ages. With increased work challenges, the stress levels are beginning to rise for almost everyone. It has become an emerging issue in health care industry as it is affecting the efficiency of men and women employees. This paper addresses work-life balance across genders. The main focus of the study was men and women employees working in hospitals. A sample of 115 nurses and doctors and other staff was taken. The purpose of this study is to find out the gender differences in the perception of work life balance and to find out the factors that will help employees in maintaining work-life balance. In the study, work and family related variables were identified and analysed with the help of Chi square test. The study concludes that men and women genders have strong belief that flexible working hours will enhance the work life balance. Work has an adverse effect on both men and women, as it affected their health and even their sleep. The analysis of spill over of work into family life showed that both men and women did not have enough time to spend with their families and moreover work pressures affected quality the of family life. Both genders opinion that a positive work environment is needed to work life balance.