The objective of the present study was to elucidate the peculiar features of the distribution of N-ethyl-3-hydroxy-2-phenyl-N-(piridin-4-yl-methyl)propanamide (tropicamide) in the body of warm-blooded animals (rats) after its intragastric administration. The following methods were employed in the study: TLC, chromogenic reactions, electronic spectrophotometry, and GC-MS. The results of the quantitative analysis of N-ethyl-3-hydroxy-2-phenyl-N-(piridin-4-yl-methyl)propanamide in different organs of warm-blooded animals (rats) were compared within 20 minutes, 2.5 and 6 hours after its single intragastric administration at a dose equivalent to 1.5 LD50 of the toxic substance. It was shown that at all time intervals the maximum amount of tropicamide was present in the tissues of the stomach. Small intestines, their contents, brain, lungs, and spleen.

We have studied the specific features of the distribution of 2,6-di-tret-buthyl-4-methlhydroxybenzole in the organism of the omnivorous warm-blooded animals (rats) following the intragastric administration of the three-fold lethal dose of the poisonous substance. 2,6-di-tret-buthyl-4-methlhydroxybenzole was isolated from the blood and various organs of the animals by means of acetone extraction. It was further purified on the 40/100 mcm L silicagel column with the use of hexane/acetone for elution. TLC, GC-MS, and UV-spectrophotometry were employed to identify and quantify the material of interest. It was shown that 2,6-di-tret-buthyl-4-methlhydroxybenzole undergoes modification in the internal organs and blood of the poisoned animals. In was present in the highest amounts (100 mg/g) in the stomach and small intestine contents (787.78±52.31 and 18.31±3.47 respectively), spleen (12.46±1.02), and kidneys (8.48±0.61).

This work was designed to study the distribution of 4-metoxyhydroxybenzene in the organism of the omnivorous warm-blooded animals (rats) after the intragastric administration of this poisonous compound at a dose three-fold greater than the LD50 value. The administered 4-metoxyhydroxybenzene was isolated from the organs and blood of the experimental animals by exposing the biological tissues to acetone with subsequent purification on a silica gel L 40/100 mcm using a hexane:dioxane:propanol-2 (20:5:1) as the mobile phase. The identification and quantitation of 4-metoxyhydroxybenzene were carried out with the use of TLC, GC-MS, and UF-spectrophotometry. It was shown that the administered 4-metoxyhydroxybenzene remained unmetabolized in the internal organs and blood of the poisoned experimental animals. The largest amounts of 4-metoxyhydroxybenzene were found in the stomach contents (2584,92±117,47), brain (59.49±6.05), contents of small intestines (28.21±3.77), and kidneys (26.13±1.64).

The objective of the present work was to study the specific distribution patterns of carbosulfan in the body of warm-blooded animals by means of thin-layer chromatography, low-pressure column chromatography, electron spectrophotometry, and GCh/ MS. The carbosulfan distribution in the warm-blooded animals (rats) was investigated after the intragastric administration of this poisonous substance at a dose equivalent to three median lethal doses (LD50) in the form of an aqueous emulsion. The highest concentrations of carbosulfan were found in the contents of the stomach, small and large intestines, liver, and lungs. The same organs and spleen contained carbofuran, the main metabolite of carbosulfan.

The objective of the present study was to develop the universal approach to the quantitative determination of acetylsalicylic acid in biological tissues and fluids to be applied in the practice of forensic chemical expertise with the use of thin-layer chromatography, gas chromatography and mass spectrometry, low-pressure column chromatography, and spectrophotometry. A system of solvents consisting of acetone and ethyl acetate (7:3) was proposed as a universal agent for extracting acetylsalicylic acid from the cadaveric tissues and blood. It was shown that acetylsalicylic acid and its principal metabolite, salicylic acid, can be purified from the endogenous admixtures present in the biological materials by column chromatography on silica gel L 40/100 mcm. Salicylic acid in extracts from biological materials was identified and quantified with the use of thin-layer chromatography, gas chromatography/mass spectrometry, and electronic spectrophotometry. The method for forensic chemical investigation of acetylsalicylic acid has been developed and applied in the analysis of the material provided for expertise.