No abstract

In this paper, we present a Fourier Transform micro-spectrometer which works based on a lamellar grating interferometer. The spectrometer model is electrostatically driven by parallel plate actuators which change the optical path difference (OPD) between movable grating facets and fixed ones. With a 200V input voltage, a maximum OPD of 48.7μm is achieved. The spectrum of a combined light source of a diode-pumped solid-state (DPSS) laser (λ=532.0nm) and a laser diode (λ=637.2nm) is experimentally acquired to demonstrate the performance of the model. The reconstructed spectrum displays two separate spectral peaks at 530.5nm and 635.2nm and the corresponding full-width at half-maximum (FWHM) resolutions are 9.8nm and 12.8nm respectively, indicating good wavelength accuracy and optical resolution.

The present study aims to use polymer dots to explore whether they can visualize tumor lesions in a diethylnitrosamine (DENA)-induced hepatocellular carcinoma (HCC) model. The HCC rat model was set up, and serum liver function indexes and AFP were tested on days 0, 30, 60, and 90 of the modeling process. After characterization of the polymer dots, they were injected into the rats and mice. The liver, spleen, and kidney of rats and the gallbladder of mice were extracted to verify the metabolic pathways of the polymer dots and their capability of fluorescent localization of HCC and gallbladder by fluorescence imaging. Strong fluorescent emission from the liver appeared immediately and 15 min after the polymer dots were injected through the main portal veins and tail veins of the model rats, respectively. A satisfactory fluorescent imaging effect lasted up to 45 min. Polymer dots circulate through the bloodstream within intrahepatic vessels rather than intracellular areas and can be clearly visualized by using both the pCLE and IVIS spectrum imaging systems. Contrast imaging of HCC lesions without fluorescent emissions was due to the lack of normal portal-hepatic veins within the tumor areas. Fluorescent imaging of the gallbladder could also be detected at 15 min after the polymer dots were injected through the tail veins of mice. The polymer dots had satisfactory fluorescent localization capability for targeted intrahepatic vessels and HCC lesions in vivo and showed potential practical value in hepato-biliary surgery.