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Histiocytic sarcomas are characterized by proliferation and/or infiltration of neoplastic histiocytes localized to specific organs, unlike malignant histiocytosis which involves many organ systems. Only a few cranial histiocytic sarcomas have been reported. Here we describe four dogs that presented with neurological deficits referable to the forebrain, and were diagnosed histologically as having histiocytic sarcoma. Using magnetic resonance (MR) imaging, the tumors were characterized by a T2-hyperintense and T1-isointense mass in one dog, T2- and T1-isointense extraaxial masses in two dogs, and a diffuse T2-hyperintense lesion over the left cerebral cortex in one dog. All tumors had contrast enhancement. MRI features in three of the four dogs were similar to that of meningioma, supported by the observation of a dural tail in two of these three dogs, and a broad base of attachment in the other. In the other dog the imaging findings were similar to those of encephalitis. Intracranial histiocytic sarcoma does not appear to have specific MR imaging features and can be confused with meningioma or encephalitis.

We analyzed DNA from 63 Japanese men with either azoospermia or severe oligospermia whose Y chromosomes were cytogenetically normal. A total of 16 loci were examined: 15 loci on the long arm between DYS7E and DYZ1, and the YRRM1 locus, a candidate gene for the azoospermic factor, AZF. One patient with a pericentric inversion of the Y chromosome was also included. We detected micro-deletions in ten individuals. The YRRM1 gene was involved in only three of them. The remaining seven patients showed deletion between DYS7C and DYS239 in common, indicating the presence of at least one additional gene, deletion of which causes azoospermia.

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ABSTRACT. A mass lesion in the subependymal region of the lateral ventricle in a 13-year-old neutered male mongrel cat with a complaint of somnolence, right circling movement and posture abnormality was examined. The magnetic resonance image examination revealed a relatively large T1-hypointense and T2-hyperintense mass lesion in the left interventricular foramen region, and there were no abnormalities in the chest and abdominal x-ray radiographic, funduscopic, and electric retinogram findings. The cat was died 43 days after the initial referral, and the post-mortem examinations revealed a poorly demarcated subependymal mass. Histologically, the brain lesion consisted of complex proliferation of highly pleomorphic cells resembling histiocytes with atypia and abundant mitotic figures. Moderate infiltrates of small reactive lymphocytes were admixed with the pleomorphic cell population. Gemistcytic astrocytes were also intermingled with the periphery of neoplastic foci. Immunohistochemically, most of the pleomorphic cells were positive for HLA-DR alphachain and ionized calcium binding adaptor molecule 1, and few were positive for lysozyme and alpha-1 antichymotrypsin. The atypical pleomorphic cells were negative for CD3, IgG (H and L), glial fibrillary acidic protein and neurofilament, suggesting monocytic/histiocytic-origin of the cells. The number of Ki-67-positive cell nuclei was extremely large, reflecting the high growth activity of these cells. Based on the findings, the lesion was considered as histiocytic sarcoma.KEY WORDS: brain, feline, histiocytic sarcoma, immunohistochemistry.J. Vet. Med. Sci. 72(1): 99-102, 2010 In dogs, histiocytic disorders have been classified into 3 major categories: canine cutaneous histiocytoma; canine reactive histiocytoses (both cutaneous and systemic); and histiocytic sarcoma complex (both localized and disseminated histiocytic sarcoma) [10]. Cats, in contrast, are rarely affected by these diseases [6]. Histiocytic sarcoma (HS) in cats has been reported to date in the intestinal wall, femur, tarsus, spleen and liver, and mediastinal and vertebral canals [1,5,11,13,16]. A brief description of an HS in the central nervous system (CNS) of a cat was reported only in a review article [19], while malignant histiocytic disorders have been well demonstrated in the canine CNS [2,4,14,18,20]. This report documents the features of a brain tumor in a cat. The histological and immunohistochemical natures characterized by the proliferation of highly pleomorphic cells resembling histiocytes and immunoreactivity for HLA-DR and ionized calcium binding adaptor molecule 1 (Iba1) are consistent with HS [7,8,15].A 13-year-old neutered male mongrel cat was presented to a private animal hospital with a complaint of somnolence, blindness, right circling movement and posture abnormality. The neurological examinations revealed a postural reaction decrease, a left mydriasis and disappearance of a physiologic nystagmus. Complete blood cell count and serum biochemical analyses were within normal ranges...