Biohybrid consists of a living organism or cell and at least one engineered component. Designing robot–plant biohybrids is a great challenge: it requires interdisciplinary reconsideration of capabilities intimate specific to the biology of plants. Envisioned advances should improve agricultural/horticultural/social practice and could open new directions in utilization of plants by humans. Proper biohybrid cooperation depends upon effective communication. During evolution, plants developed many ways to communicate with each other, with animals, and with microorganisms. The most notable examples are: the use of phytohormones, rapid long-distance signaling, gravity, and light perception. These processes can now be intentionally re-shaped to establish plant–robot communication. In this article, we focus on plants physiological and molecular processes that could be used in bio-hybrids. We show phototropism and biomechanics as promising ways of effective communication, resulting in an alteration in plant architecture, and discuss the specifics of plants anatomy, physiology and development with regards to the bio-hybrids. Moreover, we discuss ways how robots could influence plants growth and development and present aims, ideas, and realized projects of plant–robot biohybrids.

Gamma secretase is a multi-subunit complex with aspartic intramembrane protease activity that is involved in the pathogenesis of Alzheimer's disease in humans. In Arabidopsis thaliana, γ-secretase subunits are localized to endomembrane system compartments and interact with each other in a similar manner to their human counterparts. Here, we identified the protein partners of two plant γ-secretase subunits, presenilin 2 and PEN-2, by tandem affinity purification and co-immunoprecipitation, respectively. Integral membrane proteins were found to interact with presenilin 2, whereas secreted proteins were found to interact with PEN-2. Microscopy screening revealed that the reticulon family protein, RTNLB1, and two single transmembrane domain proteins, TIR-X and the phytolongin PHYL1.1, interact with presenilins. Finally, we show that RTNLB1 interacts with TIR-X. These results represent a step toward elucidating the functions of γ-secretase subunits in plant cells. Keywords Gamma-secretase • Arabidopsis proteins • Presenilins • Protein-protein interactions • Endomembrane proteins Abbreviations APH-1 Anterior pharynx defective 1 APP Amyloid precursor protein Co-IP Co-immunoprecipitation FLS2 Flagellin sensitive 2 FRET-FLIM Förster resonance energy transfer by fluorescence lifetime imaging GFP Green fluorescence protein MEF Mouse embryonic fibroblast PEN-2 Presenilin enhancer 2 Ps Presenilin PSDKO Presenilin double knockout RFP Red fluorescence protein SNARE Soluble NSF(N-ethylmaleimide-sensitive factor) attachment protein) receptor TAP Tandem affinity purification TIR Toll/interleukin-1 receptor homology domain TMD Transmembrane domain Communicated by Z. Zhang.