Five strains of Streptomyces were earlier reported to have potential for charcoal rot control and plant growth promotion (PGP) in sorghum. In this study, those five Streptomyces strains were characterized for their enzymatic activities and evaluated for their PGP capabilities on rice. All the Streptomyces were able to produce lipase, β-1,3-glucanase, grew in NaCl (up to 8%), at pH 5−13, temperatures 20−40 o C and were resistant to ampicillin, sensitive to nalidixic acid and highly sensitive to chloramphenicol, kanamycin, streptomycin and tetracycline. They were highly tolerant to fungicide bavistin, whereas highly sensitive to benlate, benomyl and radonil. When evaluated on rice in the field, the Streptomyces significantly enhanced tillers, panicles, stover yield, grain yield, dry matter, root length, volume and dry weight over the control. In the rhizosphere at harvest, microbial biomass carbon and nitrogen, dehydrogenase activity, total N, available P and % organic carbon were also found significantly higher in Streptomyces treated plots over the control. This study further confirms that the selected Streptomyces have PGP activities.