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Standardization of Siddha formulations is a major step for establishment of biological activity, consistent chemical profile, or quality control for production and manufacturing of herbal drugs. Urai Mathirai (UM) is a Siddha polyherbal preparation comprising of Chukku (Zingiber officinale Roscoe), Adimathuram (Glycyrrhiza glabra L.), Akkirakaram (Anacyclus pyrethrum (L.) Lag.), Vashambu (Acorus calamus L.), Catikkai (Myristica fragrans Houtt.), Katukkai (Terminalia chebula Retz.), Masikkai (Quercus infectoria G.Olivier), Acanam (Allium sativum L.), Tippili (Piper longum L.) and Perunkayam (Ferula aasa-foetida L.). UM reduces accumulated Aiyam with ingredients having hot potency which increases Azhal. As UM is an important medicine in Siddha pediatrics the current study is aimed to standardize UM employing standard testing protocol for AYUSH drugs. Macroscopic authentication, powder microscopy and physico‐chemical studies like loss on drying, water soluble ash, acid insoluble ash, ethanol soluble extractive, water soluble extractive, pH and HPTLC were performed as per standard methodology. The current study derived quality indicating botanical and chemical fingerprints routine quality check of UM