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Exergy analysis is carried out on a single-cylinder CI engine fueled with biodiesel blends of palm, jatropha and cottonseed oils. This is to identify the blends with high exergy destruction. To this end, experimental and analytical methods were adopted. Three types of biodiesel blends incorporated in this study are primary, binary and ternary. The load was varied as an independent parameter, and mass flow rates of air and fuel, flue gas composition, etc., were measured during the study. Moreover, the chemical composition of the fuel blends and flue gas, as well as their flow rates, were used to determine the total exergy. The output parameters determined were 1st and 2nd law efficiency and fuel exergy destruction under all loading conditions. The inference obtained from the experiment suggests minutely higher 1st law efficiency for the biodiesel blends. Increasing the blending ratio led to an increase in efficiency indices.