Objective:To evaluate the effect of final rinsing solution on smear layer removal through penetrability of the root canal sealer.Materials and Methods:Each of the 30 teeth selected was decoronated and the root length standardized at 16 mm measured from the apex of the tooth to the cementoenamel junction (CEJ). A size 10-k file was used and working length was determined by subtracting 1mm from the measured length so that the length of the sample was 15mm. A combination of step back and crown down technique was done and apical portion was prepared till H-file no. 30. 3% NaOCl was used during cleaning and shaping of the canals. Finally the teeth were divided into three groups of 10 teeth each. Group-I was irrigated with 17% EDTA, Group-II with TUBLICID plus, and Group-III with Biopure MTAD®. ISO 30 size gutta-percha points were selected as master cones. The Acroseal sealer was mixed with approximated concentration 0.1% fluorescent rhodamine B isothiocyanate dye. Obturation was done and after 48 hours, the roots were sectioned and subjected to confocal laser scanning microscopy.Results:Comparisons among the three groups were done by two-way analysis of variance (ANOVA) and Newman-Keuls multiple post-hoc procedures. The two-way ANOVA showed statistically highly significant results. The maximum depth of sealer penetration was observed in Group II followed by Group III and Group I.Conclusion:The use of -Tubulicid plus as a final irrigant provided better smear layer removal than Biopure a mixture of doxicycline, citric acid and a detergent (Tween 80) (MTAD®) and 17% EDTA.

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C glabrata was the most commonly found species among denture wearers and non-denture wearers both by conventional and CHROMagar methods. In males, C. albicans was the predominant species, whereas C. glabrata was the predominant species in females. Candidal colonization was higher in denture wearers compared to non-denture wearers, especially among females. The CHROMagar method was more rapid compared to conventional methods. In the present study, CHROMagar Candida showed 100% specificity and 100% sensitivity when compared to SDA and conventional methods.

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Introduction and Aim: Toothpastes are the most common preventive means in oral health care. Many commercially available dentifrices claim to have antimicrobial properties, but little research has been conducted to investigate these claims. Therefore, this study was conducted to evaluate the efficacy of different toothpaste formulations in reducing the oral microbial load. Methodology: A total of six types of tooth pastes-4 herbal and 2 non-herbal- were assigned to a group of 20 subjects each, who used it for a period of six weeks. During the course of the study, at the end of 1st, 3rd and 6th week, subgingival plaque samples collected were subjected to microbial analysis. Results: Microbial analysis identified colonies of organisms such as S. mitis, S. mutans, S. salivarius, Peptostreptococcus species, Prevotella species and Lactobacillus species. Colony forming units (cfu) calculated indicated a reduction in microbial load from 1st to 6th week of use of different tooth paste formulations. However statistical analysis of the results did not show significant differences between the groups (p>0.005). Conclusion: The selected tooth paste formulations were effective in controlling the microbial load and therefore contributing to maintain good oral hygiene. However, practicing appropriate oral hygiene measures & brushing technique is of utmost importance in maintaining good oral health than the effectiveness of various ingredients in the toothpastes used.