No abstract

Numerous new taxa and classifications of Dothideomycetes have been published following the last monograph of families of Dothideomycetes in 2013. A recent publication by Honsanan et al. in 2020 expanded information of families in Dothideomycetidae and Pleosporomycetidae with modern classifications. In this paper, we provide a refined updated document on orders and families incertae sedis of Dothideomycetes. Each family is provided with an updated description, notes, including figures to represent the morphology, a list of accepted genera, and economic and ecological significances. We also provide phylogenetic trees for each order. In this study, 31 orders which consist 50 families are assigned as orders incertae sedis in Dothideomycetes, and 41 families are treated as families incertae sedis due to lack of molecular or morphological evidence. The new order, Catinellales, and four new families, Catinellaceae, Morenoinaceae Neobuelliellaceae and Thyrinulaceae are introduced. Seven genera (Neobuelliella, Pseudomicrothyrium, Flagellostrigula, Swinscowia, Macroconstrictolumina, Pseudobogoriella, and Schummia) are introduced. Seven new species (Acrospermum urticae, Bogoriella complexoluminata, Dothiorella ostryae, Dyfrolomyces distoseptatus, Macroconstrictolumina megalateralis, Patellaria microspora, and Pseudomicrothyrium thailandicum) are introduced base on morphology and phylogeny, together with two new records/reports and five new collections from different families. Ninety new combinations are also provided in this paper.

Strigula sensu lato has been previously defined based on phenotype characters as a rather broad genus including tropical to temperate species growing on a wide array of substrata. In this study, based on a multilocus phylogenetic approach, we show that foliicolous species form six well-delimited clades that correlate with diagnostic phenotype features, including thallus morphology, carbonization of the involucrellum and excipulum, ascospore dimensions, and type of macroconidia. Given the topology, with five of the six clades emerging on long stem branches, and the strong phenotypical differentiation between the clades, we recognize these at the genus level, making mostly use of previously established genus names. Four genera, namely Phylloporis, Puiggariella, Raciborskiella, and Racoplaca, are resurrected for the S. phyllogena, S. nemathora, S. janeirensis, and S. subtilissima groups, respectively, whereas one new genus, Serusiauxiella gen. nov., is introduced for a novel lineage with peculiar macroconidia. The only sequenced non-foliicolous species, S. jamesii, is not closely related to these six foliicolous lineages but clusters with Flavobathelium and Phyllobathelium, revealing it as an additional undescribed genus-level lineage being treated elsewhere. Within the new genus Serusiauxiella, three new species are described: Serusiauxiella filifera sp. nov., S. flagellata sp. nov., and S. sinensis sp. nov. In addition, ten new combinations are proposed: Phylloporis austropunctata comb. nov., P. radiata comb. nov., P. vulgaris comb. nov., Puiggariella confluens comb. et stat. nov., P. nemathora comb. nov., P. nigrocincta comb. nov., Racoplaca maculata comb. nov., R. melanobapha comb. nov., R. transversoundulata, and R. tremens comb. nov. We also report on a peculiar, previously unrecognized growth behaviour of the macroconidial appendages in Strigula s.lat.