Improved understanding of the molecular mechanisms and immunoregulation of muscle-invasive bladder cancer (MIBC) is essential to predict prognosis and develop new targets for therapies. In this study, we used the cancer genome atlas (TCGA) MIBC and GSE13507 datasets to explore the differential co-expression genes in MIBC comparing with adjacent non-carcinoma tissues. We firstly screened 106 signature genes by Weighted Gene Co-expression Network Analysis (WGCNA) and further identified 15 prognosis-related genes of MIBC using the univariate Cox progression analysis. Then we systematically analyzed the genetic alteration, molecular mechanism, and clinical relevance of these 15 genes. We found a different expression alteration of 15 genes in MIBC comparing with adjacent non-carcinoma tissues and normal tissues. Meanwhile, the biological functions and molecular mechanisms of them were also discrepant. Among these, we observed the ANLN was highly correlated with multiple cancer pathways, molecular function, and cell components, revealing ANLN may play a pivotal role in MIBC development. Next, we performed a consensus clustering of 15 prognosis-related genes; the results showed that the prognosis, immune infiltration status, stage, and grade of MIBC patients were significantly different in cluster1/2. We further identified eight-genes risk signatures using the least absolute shrinkage and selection operator (LASSO) regression analysis based on the expression values of 15 prognosis-related genes, and also found a significant difference in the prognosis, immune infiltration status, stage, grade, and age in high/low-risk cohort. Moreover, the expression of PD-1, PD-L1, and CTLA4 was significantly up-regulated in cluster1/high-risk-cohort than that in cluster2/low-risk-cohort. High normalized enrichment score of the Mitotic spindle, mTORC1, Complement, and Apical junction pathway suggested that they might be involved in the distinct tumor immune microenvironment (TIME) of cluster1/2 and high-/low-risk-cohort. Our study identified 15 prognosis-related genes of MIBC, provided a feasible stratification method to help for the future immunotherapy strategies of MIBC patients.

Martial arts education has a relatively comprehensive educational function. Compared with other educational methods, it has some unique features. When martial arts education carries out moral education, it not only attaches importance to the teaching of moral norms but also requires martial arts practitioners to practice moral norms, so martial arts education is more practical in improving moral literacy. In fact, the role of martial arts education is far from just playing its role in strengthening the body. This kind of prejudice of mindset conceals the diversity characteristics of martial arts education. This paper proposes to apply artificial intelligence technology in martial arts education governance, which uses the target tracking algorithm based on deep learning to track and analyze the movement of martial arts practitioners. At the same time, this paper uses the pose estimation algorithm of coordinate regression to predict the key points of the human body from the global perspective of the human body and then locates the key points of the human body from the features. It greatly simplifies the prediction of key points and solves the problem of nonstandard movements of students in martial arts education. The experimental analysis part includes the results and analysis of the impact of AI-based flipped classroom teaching on students’ martial arts learning and the comparison and analysis of students’ martial arts learning in the two classes after the experiment. The analysis results show that the P values of the four aspects of learning interest, active participation attitude, independent exploration ability, and analysis and problem-solving ability of the two classes are all less than 0.01, indicating that there is a significant difference.