No abstract

Ocean warming can cause injury and death in mussels and is believed to be one of the main reasons for extensive die-offs of mussel populations worldwide. However, the biological processes by which mussels respond to heat stress are still unclear. In this study, we conducted an analysis of enzyme activity and TMT-labelled based proteomic in the digestive gland tissue of Mytilus coruscus after exposure to high temperatures. Our results showed that the activities of superoxide dismutase, acid phosphatase, lactate dehydrogenase, and cellular content of lysozyme were significantly changed in response to heat stress. Furthermore, many differentially expressed proteins involved in nutrient digestion and absorption, p53, MAPK, apoptosis, and energy metabolism were activated post-heat stress. These results suggest that M. coruscus can respond to heat stress through the antioxidant system, the immune system, and anaerobic respiration. Additionally, M. coruscus may use fat, leucine, and isoleucine to meet energy requirements under high temperature stress via the TCA cycle pathway. These findings provide a useful reference for further exploration of the response mechanism to heat stress in marine mollusks.

Senescence and death occur after the spawn of female cuttlefish, which results in an immense loss of reproductive resources. To better understand the physiological mechanisms involving death after spawning in Sepiella japonica (Japanese spineless cuttlefish). We used proteomic methods to identify differentially expressed proteins (DEPs) in the optic glands of S. japonica at three distinct growth stages: pre-spawning after sexual maturity (group A); spawning (group B) and post-spawning before death (group C). About 955, 1000, and 1024 DEPs were identified for each comparative group analysis (i.e. group B vs A, group B vs C, and group C vs A). The gene ontology (GO) analysis showed that the function of DEPs was correlated with peptidase activity, metal-ion binding and structural molecule activity, and the majority of DEPs were involved in signal transduction and energy metabolism. It was also identified some aging-related pathways in the results of KEGG annotation. Based on previous studies of anti-aging effects in cephalopods, our study provides a new insights about the death of S. japonica after spawning at the protein level.

To better understand the physiological events involving death after spawning in S. japonica (Japanese spineless cuttlefish), we have presently generated a proteomic data set to properly examine this phenomenon. As such, a proteomic-based approach was employed to identify differentially expressed proteins (DEPs) in the optic glands of S. japonica, at three distinct growth stages: pre-spawning after sexual maturity (group A); spawning (group B) and postspawning before death (group C). About 955, 1000, and 1024 DEPs were identified for each comparative group analysis (i.e., group B vs A, group B vs C, and group C vs A). We further discovered that the function of these newly identified DEPs was mostly related to molecular events such as gene translation and signal transduction. According to the enriched GO terms obtained by Gene Ontology analysis, the function of most DEPs was correlated with structural molecule activity, ribosome function and gene expression. The majority of DEPs were known to be involved in signal transduction and energy metabolism, interestingly, some aging-related DEPs were also identified. Putting together, our study provides new insights, at the protein level, in the phenomenon of death after spawning in S. japonica, by referring to anti-aging effects conserved in other cephalopoda species.

To better understand the physiological events involving death after spawning in S. japonica (Japanese spineless cuttlefish), we have presently generated a proteomic data set to properly examine this phenomenon. As such, a proteomic-based approach was employed to identify differentially expressed proteins (DEPs) in the optic glands of S. japonica, at three distinct growth stages: pre-spawning after sexual maturity (group A); spawning (group B) and postspawning before death (group C). About 955, 1000, and 1024 DEPs were identified for each comparative group analysis (i.e., group B vs A, group B vs C, and group C vs A). We further discovered that the function of these newly identified DEPs was mostly related to molecular events such as gene translation and signal transduction. According to the enriched GO terms obtained by Gene Ontology analysis, the function of most DEPs was correlated with structural molecule activity, ribosome function and gene expression. The majority of DEPs were known to be involved in signal transduction and energy metabolism, interestingly, some aging-related DEPs were also identified. Putting together, our study provides new insights, at the protein level, in the phenomenon of death after spawning in S. japonica, by refering to anti-aging effects conserved in other cephalopoda species.