Aeroelasticity has become a critical issue for Multi-Megawatt wind turbine due to the longer and more flexible blade. In this paper, the development of aeroelasticity and aeroelastic codes for wind turbine is reviewed and the aeroelastic models for wind turbine blade are described, based on which, the current research focuses for large scale wind turbine are discussed, including instability problems for onshore and offshore wind turbines, effects of complex inflow, nonlinear effects of large blade deflection, smart structure technologies, and aerohydroelasticity. Finally, the future development of aeroelastic code for large scale wind turbine: aeroservoelasticity and smart rotor control; nonlinear aeroelasticity due to large blade deflection; full-scale 3D computational fluid dynamics (CFD) solution for dynamics; and aerohydroelasticity are presented.
Base editing has the potential to improve important economic traits in agriculture and can precisely convert single nucleotides in DNA or RNA sequences into minimal double-strand DNA breaks (DSB). Adenine base editors (ABE) have recently emerged as a base editing tool for the conversion of targeted A:T to G:C, but have not yet been used in sheep. ABEmax is one of the latest versions of ABE, which consists of a catalytically-impaired nuclease and a laboratory-evolved DNA-adenosine deaminase. The Booroola fecundity (FecB B) mutation (g.A746G, p.Q249R) in the bone morphogenetic protein receptor 1B (BMPR1B) gene influences fecundity in many sheep breeds. In this study, by using ABEmax we successfully obtained lambs with defined point mutations that result in an amino acid substitution (p.Gln249Arg). The efficiency of the defined point mutations was 75% in newborn lambs, since six lambs were heterozygous at the FecB B mutation site (g.A746G, p.Q249R), and two lambs were wild-type. We did not detect off-target mutations in the eight edited lambs. Here, we report the validation of the first gene-edited sheep generated by ABE and highlight its potential to improve economically important traits in livestock.
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