No abstract

A Gram-stain-negative, rod-shaped, exopolysaccharide-producing, strictly aerobic bacterium with a single polar flagellum, designated strain HL22-2 T , was isolated from a phosphate mine situated in a suburb of Kunmming in Yunnan province in south-western China. The taxonomic status of this strain was evaluated by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HL22-2 T was related to members of the genus , 2007). At the time of writing, the genus Pseudomonas comprised at least 207 species with validly published names (http://www.bacterio.net/p/pseudomonas.html). During an investigation of the diversity of cultivable micro-organisms in a phosphate mining field ecosystem, strain HL22-2 T was isolated from phosphate rock powder samples and selected for further characterization using a polyphasic approach, including analysis of genotypic, chemotaxonomic and classical phenotypic characteristics. Based on our results, we consider that strain HL 22-2 T represents a novel species of the genus Pseudomonas.Strain HL 22-2 T was isolated from phosphate rock powder samples collected at a phosphate mining field situated in a suburb of Kunmming in Yunnan province in south-western China. A sample (1 g) was suspended in 9 ml sterile distilled water and serial dilutions were made. Aliquots (0.1 ml) of appropriate dilutions were spread on Luria-Bertani (LB pH 7.2; 1 % tryptone, 1 % NaCl, 0.5 % yeast extract; w/v) agar plates and incubated at 30 u C for 4-5 days. The purity of cultures was assessed by colony morphology and microscopy. For short-term preservation, serial transfer from agar slants to an appropriate medium was performed and the agar slants were stored at 4 u C. For long-term preservation, the strain was stored at 280 u C in LB broth with 20 % glycerol (v/v). For taxonomic purposes, Pseudomonas xanthomarina KMM 1447 T and Pseudomonas stutzeri ATCC 17588 T were purchased from their respective culture collection centres and used as reference strains.Abbreviation: EPS, exopolysaccharides.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain HL22-2 T is JQ246444.

A yellow-pigmented, Gram-stain-negative, strictly aerobic, rod-shaped, round-ended bacterium, designated strain 18-11HK T , was isolated from a phosphate mine situated in the suburb of Kunming in Yunnan province in south-western China. The taxonomic status of this strain was evaluated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, strain 18-11HKT was shown to belong to the genus Novosphingobium, showing the highest levels of sequence similarity with respect to 'Novosphingobium ginsenosidimutans' FW-6 (97. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain 18-11HK

In this work, the Pb adsorption and removal ability of biochar from simulated Pb(II)-contaminated wastewater, adsorption isotherms, kinetics, and thermodynamics were studied. Adsorption characteristics of biochar on Pb(II) were analyzed by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and scanning electron microscope with energy dispersive spectrometer (SEM-EDS). The influence of the pH of the solution, the contact time, and the biochar dose on the removal of Pb(II) were investigated by single-factor design and response surface analysis. With the increase in biochar dose from 2 g/L to 4 g/L in wastewater, the Pb(II) amount adsorbed on biochar reduced from 21.3 mg/g to 17.5 mg/g. A weakly acidic environment was more conducive to the ligand exchange between Pb(II) ions and biochar. Pb(II) adsorption kinetics of biochar showed that the Pseudo-first-order model was more suitable than other employed models to describe the adsorption process. During the isothermal adsorption process, Langmuir and Freundlich’s isotherms fitted the adsorption data very well (R2 > 96%). The Pb (II) adsorption onto biochar was spontaneous in the specified temperature range (298–318 K) and the process was exothermic. Simultaneously, the optimal conditions were a pH of 5, a contact time of 255 min, and a biochar dose of 3 g/L, under which the maximum predicted Pb(II) removal efficiency was 91.52%.

A Gram-stain-negative, facultatively anaerobic, non-pigmented, non-sporulating, rod-shaped bacterial strain T ) was isolated from a phosphate mine in Yunnan Province, China.The cells were motile with a single polar flagellum. et al., 2013). During an investigation of the diversity of cultivable micro-organisms in a phosphatemining-field ecosystem, a strain (designated WYH 22-41 T ) was isolated from phosphate rock powder samples and selected for further characterization using a polyphasic approach, including analysis of genotypic, chemotaxonomic and classical phenotypic characteristics. Based on our results, we consider that the strain represents a novel species of the genus Comamonas. Strain WYH 22-41T was isolated from phosphate rock powder samples collected at a phosphate-mining field situated in a suburb of Kunming in Yunnan Province, south-western China. A sample (1 g) was suspended in 9 ml sterile distilled water and serial dilutions were made. Aliquots (0.1 ml) of appropriate dilutions were spread on Luria-Bertani (LB, pH 7.2; 1 % tryptone, 1 % NaCl and 0.5 % yeast extract, all w/v) agar plates and incubated at 30 8C for 4-5 days. The purity of cultures was assessed by colony morphology and microscopy. For short-term preservation, serial transfer from agar slants to an appropriate medium was performed and the agar slants were stored at 4 8C. Cell size and morphology of strain WYH 22-41 T after growth on LB agar for 2 days at 30 8C were studied by