Objectives: Evaluate serum bilirubin in adult healthy subjects and its correlation with white blood cells as risk factor for cardiovascular disease. Study Design: Cross- sectional study. Setting: Department of Pharmacology and Medicine, Suleman Roshan Medical College. Period: January - December 2017. Material & Methods: A sample of 100 male and 100 female adult healthy subjects were recruited for study protocol. Blood glucose, Serum creatinine, Blood lipids, liver enzyme levels, White blood cell counts and Serum bilirubin levels were analyzed. Pearson`s correlation was used for the correlation coefficient and its statistical significance for the association of serum bilirubin and white blood cells. Data variables were analyzed by statistical software SPSS (ver 21.0) at 95% CI (P ≤ 0.05). Results: Mean± SD age of male and female was found 47.02±8.42 and 48.59±7.80 years respectively (P=0.071). Serum bilirubin shows statistically significant negative correlation with blood glucose (r= - 0.257, P=0.0001) and LDLc (r= - 0.155, P=0.027) and WBC (r= - 0.871, P=0.0001). Conclusion: The present study shows the elevated serum total bilirubin levels within reference range correlated negatively with total white blood cells in adult healthy population.

Objectives: To analyze the effects of Examination stress on heart rate, blood pressure and white blood cells among medical college students. Study Design: Observational study. Setting: Department of Pathology, Indus Medical College, Tando Muhammad Khan Sindh. Period: September 2018 to April 2019. Material & Methods: 200 medical students were selected according to inclusion and exclusion criteria. Heart rate was measured manually. Systemic Blood pressure (BP) was recorded by Sphygmomanometer. Mean arterial BP was calculated as diastolic BP+1/3 of pulse pressure. Blood slides were stained with Leishman`s stain and white blood cells were counted using microscope.  Data was analyzed by using Statistical Package for Social Sciences (ver 22.0) for windows Microsoft. Results: Age of study subjects was noted as 20.23±1.23 years.  Body weight and Body mass index (BMI) were noted as 64.93±7.53 Kg (P=0.0001) and 27.5±5.3 (P=0.67). Of total 200, 109 (54.5%) were male and 91 (45.5%) were female (P=0.0001). Heart rate, Systolic, Diastolic and Mean arterial BP differed significantly before and after examination stress (P<0.05). White blood cells before examination were found as 7310.43±708.5 x103/µL compared to 8202.39±677.26 during examination stress (P=0.0001). Conclusion: Examination stress perturbs the physiological homeostasis as changes in heart rate, blood pressure and White blood cells are noted among medical students. Psychological and physical strategies for coping the stress should be considered for the young medical students.

Objective: To determine association of Serum Uric Acid levels (SUA) in subjects suffering Systemic Blood Pressure and to determine its association with systolic and diastolic blood pressure. Study Design: Cross Sectional Study. Setting: Department of Faculty of Medicine and Allied Medical Sciences, Isra University, Hyderabad, Sindh, Pakistan. Period: January 2018 to September 2019. Material & Methods: 150 diagnosed cases of systemic hypertension and 150 controls were selected though non-probability purposive sampling according to inclusion and exclusion criteria. 2 ml venous blood was taken, centrifuged and sera were used for uric acid. Data was saved in a pre- structured Performa. Statistical software (SPSS v 21.0, IBM, Incorporation, USA) of data variables was analyzed at 95% CI (P ≤ 0.05). Results: Serum uric acid in controls was 2.93±0.72 compared to cases 4.25±1.44 mg/dl (P=0.0001). Hyperuricemia was observed in 58 (38.6%) cases compared to 23 (15.3%) controls. Uric acid shows strong positive association with Systolic BP (r= 0.52*, p=0.0001) and Diastolic BP (r= 0.46**, p=0.0001). Conclusion: We found hyperuricemia in 58 (38.6%) of systemic hypertension cases. Systolic and Diastolic Blood pressure show positive association with uric acid.