Cracking is one of the main diseases of small- and medium-span reinforced concrete (RC) bridges. It is a key problem to determine the change in mechanical properties of RC beams after cracking in bridge-performance evaluation. The present study performs static loading tests on seven simply supported T-beams with different crack damage conditions. The influences of crack location, crack depth and steel-bar diameter at a prefabricated crack on the stress, deflection and crack distribution pattern of pre-cracked test beams are investigated. The failure mode and mechanism of pre-cracked beams are revealed. Based on the experimental results, a finite element model of a pre-cracked beam is developed and validated. Following this, a theoretical prediction method is proposed to calculate the ultimate load of pre-cracked RC beams. The results indicate that the direct damage to mid-span section size can significantly affect the stiffness of the RC beam. The local damage of the tensile steel section has insignificant influence on the overall stiffness of the beam. The stiffness degradation of the pre-cracked beam at the quarter span is smaller than that of the pre-cracked beam at mid-span. The strain of the T-beam section in the pre-cracked test conformed to the assumption of the flat section. The experimental observations are in good agreement with the theoretical predictions, which can provide a theoretical basis for the performance evaluation of existing RC beams.