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Abstract:In this study, enzyme activities and their relationships to organics purification were investigated in three different vertical flow constructed wetlands, namely system A (planting Pennisetum sinese Roxb), system B (planting Pennisetum purpureum Schum.), and system C (no plant). These three wetland systems were fed with simulation domestic sewage at an influent flow rate of 20 cm/day. The results showed that the final removal efficiency of Chemical Oxygen Demand (COD) in these three systems was 87%, 85% and 63%, respectively. Planting Pennisetum sinese Roxb and Pennisetum purpureum Schum. could improve the amount of adsorption and interception for organic matter in the substrate, and the amount of interception of organic matter in planting the Pennisetum sinese Roxb system was higher than that in planting the Pennisetum purpureum Schum. system. The activities of enzymes (urease, phosphatase and cellulase) in systems A and B were higher than those in system C, and these enzyme activities in the top layer (0-30 cm) were significantly higher than in the other layers. The correlations between the activities of urease, phosphatase, cellulase and the COD removal rates were R = 0.815, 0.961 and 0.973, respectively. It suggests that using Pennisetum sinese Roxb and Pennisetum purpureum Schum. as wetland plants could promote organics removal, and the activities of urease, phosphatase and cellulase in those three systems were important indicators for COD purification from wastewater. In addition, 0-30 cm was the main function layer. This study could provide a theoretical basis for COD removal in the wetland system and supply new plant materials for selection.

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