Acute lung injury (ALI), a prevalent complication of severe acute pancreatitis (SAP), is also a leading contributor to respiratory failure and even death of SAP patients. Here, we intended to investigate the function and mechanism of stellate ganglion block (SGB) in ameliorating SAP-induced ALI (SAP-ALI). We engineered an SAP-ALI model in rats and treated them with SGB. HE staining and the dry and wet method were implemented to evaluate pathological alterations in the tissues and pulmonary edema. The rats serum changes of the profiles of TNF-α, IL-6, IL-1β, and IL-10 were examined. The profiles of miR-155-5p and SOCS5/JAK2/STAT3 were detected. Functional assays were performed for confirming the role of miR-155-5p in modulating the SOCS5/JAK2/STAT3 pathway in pulmonary epithelial cells. Our findings revealed that SGB vigorously alleviated SAP rat lung tissue damage and lung edema and lessened the generation of pro-inflammatory cytokines TNF-α, IL-6, and IL-1β. SGB enhanced SOCS5 expression, hampered miR-155-5p, and suppressed JAK2/STAT3 pathway activation. As evidenced by mechanism studies, miR-155-5p targeted the 3′UTR of SOCS5 and repressed its expression, hence resulting in JAK2/STAT3 pathway activation. During animal trials, we discovered that SGB ameliorated SAP-ALI, boosted SOCS5 expression, and mitigated the levels of pro-inflammatory factors and miR-155-5p in the plasma. In vitro, miR-155-5p overexpression substantially facilitated pulmonary epithelial cell apoptosis, inflammation, and JAK2/STAT3 pathway activation and restrained SOCS5 expression. All in all, our work hinted that SGB could modulate the miR-155-5p/SOCS5/JAK2/STAT3 axis to alleviate SAP-ALI.

This paper took extra low permeability reservoirs in Dagang Liujianfang Oilfield as an example and analyzed different types of microscopic pore structures by SEM, casting thin sections fluorescence microscope, and so on. With adoption of rate-controlled mercury penetration, NMR, and some other advanced techniques, based on evaluation parameters, namely, throat radius, volume percentage of mobile fluid, start-up pressure gradient, and clay content, the classification and assessment method of extra low permeability reservoirs was improved and the parameter boundaries of the advantageous reservoirs were established. The physical properties of reservoirs with different depth are different. Clay mineral variation range is 7.0%, and throat radius variation range is 1.81 m, and start pressure gradient range is 0.23 MPa/m, and movable fluid percentage change range is 17.4%. The class IV reservoirs account for 9.56%, class II reservoirs account for 12.16%, and class III reservoirs account for 78.29%. According to the comparison of different development methods, class II reservoir is most suitable for waterflooding development, and class IV reservoir is most suitable for gas injection development. Taking into account the gas injection in the upper section of the reservoir, the next section of water injection development will achieve the best results.

Angioneurotic headache is a common headache type in clinical treatment. At present, patients with nervous headache are mainly treated with oral western medicine in clinic, but it is usually difficult to obtain the ideal effect. In this study, we analyzed the effects of continuous lidocaine infusion through an ultrasound-guided cervical sympathetic ganglia (SG) catheter on cerebral hemodynamics and thermal imaging characteristics of head and neck in patients with angioneurotic headache and explored the clinical feasibility of this scheme. The results show that continuous infusion of lidocaine under ultrasound-guided SG catheterization can alleviate headache in patients with angioneurotic headache, which may be related to improving cerebral hemodynamics.

We examined the effect of calorie intake via enteral nutrition on diaphragmatic thickness and prognosis of mechanically ventilated patients with chronic obstructive pulmonary disease. Patients diagnosed as malnutrition were divided into observation and control groups. Both groups received conventional therapy and enteral nutrition. In the observation group, early adequate nutritional therapy was given, and the target calorie was gradually achieved within 1–3 days. In the control group, trophic feeding was provided, and the target calorie was gradually obtained after 7 days. Baseline clinical data were collected upon admission. Infection and inflammation indices, nutritional indices, and diaphragm indices were compared. Prognostic indices were compared, and the correlations of nutritional indices with diaphragmatic thickness and prognostic indices were analyzed using Pearson's analysis. The two groups had similar gender, age, heart rate, mean arterial pressure, arterial partial pressure of oxygen, acute physiology and chronic health evaluation II score, systemic inflammatory response syndrome score, multiple organ dysfunction (MODS) score, inflammation indices, nutritional indices, and diaphragmatic thickness indices upon admission (P › 0.05). Compared with values before enteral nutrition, the levels of procalcitonin, tumor necrosis factor-α, interleukin-6, and interleukin-8 significantly declined after treatment in both the groups, but greater decrease in the observation group (P ‹ 0.05). Diaphragmatic thickness at the end of expiration had no significant difference between the two groups at each time point, but diaphragmatic thickness at the end of inspiration and diaphragmatic thickening fraction significantly increased on the 7th day of enteral nutrition and after weaning, which were more significant in the observation group (P ‹ 0.05). No significant difference was found in the length of stay in intensive care unit between the two groups (P › 0.05), while the duration of mechanical ventilation, weaning success rate within 14 days, and morbidity and mortality rates of ventilator-associated pneumonia had significant differences (P ‹ 0.05). Nutritional indices were positively correlated with diaphragmatic thickness at the end of inspiration, diaphragmatic thickening fraction, duration of mechanical ventilation, and morbidity and mortality rates of ventilator-associated pneumonia, but negatively correlated with the weaning success rate within 14 days. Different enteral nutrition regimens can significantly improve the nutritional status and diaphragmatic function, inhibit the inflammatory response, shorten the duration of mechanical ventilation, and enhance the clinical treatment and prognosis effect on mechanically ventilated patients with chronic obstructive pulmonary disease

No abstract