Background: Stress, Anxiety and Depression decrease the academic performance of the students and this plays an important role in academics. The Scale used is Depression, Anxiety and Stress Scale – 21. It is a 21 Items scale which helps to find out levels of depression, anxiety and stress in an individual. It assesses difficulty relaxing, nervous arousal, and being easily upset / agitated, irritable / over-reactive and impatient. Scores for depression, anxiety and stress are calculated by summing the scores for the relevant items. Aim: To Prevalence of Depression, Anxiety and Stress Levels on DASS21 Scale in Medical Students of First Year Odd and Even Batch at Miraj City. Method: The sample size comprises of 184 students of first year from Medical Courses in Miraj including both Males and females of odd (92) and even (92) batch. The outcome measures used is a Depression anxiety and stress Scale – 21 which will be given to the students and explained in a language comfortable to them. The students will then complete the scale correctly and truly. Data will be analyzed based on the scores of each student. Outcome Measure: Depression, Anxiety and Stress Scale – 21 Result: The result proves that presence of Depression, Anxiety and Stress were seen clinically and statistically significant in intra-batches. The mean value of depression, anxiety and stress indicated mild depression in even Batch whereas moderate to severe in Odd Batch. Even batch has mild to moderate anxiety and severe to extremely severe is seen in Odd batch. Stress observed is mild to moderate in odd as well as in even batch. Conclusion: On comparison in both batches depression is found in odd batch with significant p-value, whereas anxiety and stress do not have significant p-value hence the can be compared in terms of severity levels. The study concludes that there is more depression, anxiety and stress in odd batch than in comparison with the even batch. Key words: Depression, Anxiety and Stress Scale – 21, First Year Odd and Even Batch Medical Students, mental health. .