No abstract

The effects of 3 ethanol levels (30, 50, and 70%) with and without thiamine dilaurylsulfate ( TDS ; 1,000 ppm) were evaluated for the reduction of natural mesophilic aerobic bacteria ( MAB ), coliforms, and inoculated Salmonella Typhimurium ( S. Typhimurium) in chicken skin. The chicken skin was inoculated with a 7 log cfu/mL suspension of S. Typhimurium. Loosely, intermediately, and tightly attached cells were recovered from chicken skin through shaking at 200 rpm for 5 min, stomaching for 1 min, and blending for 1 min, respectively. Increasing the ethanol concentration reduced the number of MAB, coliforms, and S . Typhimurium on the chicken skin, whereas TDS treatment without ethanol was not effective. Intermediately and tightly attached microorganisms (total MAB, coliforms, and S. Typhimurium) were more resistant to chemical disinfectants than loosely attached microorganisms. The combination of 70% ethanol with TDS was most effective than the combination of TDS with lower concentrations of ethanol in reducing populations of loosely, intermediately, and tightly attached MAB (by 1.88 log cfu/g, 1.21 log cfu/g, and 0.84 log cfu/g, respectively), coliforms (by 1.14 log cfu/g, 1.04 log cfu/g, and 0.67 log cfu/g, respectively), and S . Typhimurium (by 1.62 log cfu/g, 1.72 log cfu/g, and 1.27 log cfu/g, respectively). However, the chicken skin treated with higher concentrations of ethanol was tougher ( P < 0.05) and more yellow and less red ( P < 0.05) than that treated with lower concentrations of ethanol or with water (control). On the other hand, a combination of 30% ethanol and TDS yielded the best results, showing the reduction greater than 0.5 log cfu/g in S. Typhimurium, with no negative effect on chicken skin color or texture. Thus, a combination of 30% ethanol and TDS appears to be the optimal treatment for reducing microbial contamination of skin-on chicken products to enhance poultry safety without decreasing food quality, and this treatment could be applied in the poultry industry.

The pseudofruit of A. othonianum Rizzini, “Cerrado” cashew pulp, has been described as rich in flavonoids, phenolic compounds, and vitamin C. The objective of this work was to evaluate the beneficial health effects seen with the addition of “Cerrado” cashew pulp (CP) to an obesogenic high fat diet provided to C57BL/6J male mice. In week 9, the HF-fed group had a significantly higher baseline glucose concentration than the LF- or HF+CP-fed groups. In RNAseq analysis, 4669 of 5520 genes were found to be differentially expressed. Among the genes most upregulated with the ingestion of the CP compared to HF were Ph1da1, SLc6a9, Clec4f, and Ica1 which are related to glucose homeostasis; Mt2 that may be involved steroid biosynthetic process; and Ciart which has a role in the regulation of circadian rhythm. Although “Cerrado” CP intake did not cause changes in the food intake or body weight of fed mice with HF diet, carbohydrate metabolism appeared to be improved based on the observed changes in gene expression.

Salmonella is one of the common foodborne bacteria, causing 80.3 million illnesses every year worldwide. This study was conducted to isolate and identify Salmonella enterica serovars from poultry samples responsible for causing foodborne poisoning in the Mississippi area, United States. A total of 55 S. enterica serovars—Enteritidis (6), Oranienburg (1), Schwarzengrund (8), Heidelberg (4), Kentucky (22), 4, [5], 12:i:- (1), Montevideo (2), Infantis (9), and multi serotypes (2)—were isolated from approximately 110 poultry samples. Through pulsed-field gel electrophoresis ( PFGE ) analysis, 8 to 13 bands were obtained. The profiles showed >90% similarity in strains within the same type. Consequently, PFGE could be a useful tool to determine chromosomal similarity (clonality of strains) that can be used to trace down epidemiologic sources and geographical origins of Salmonella .

Objectives Grapes are nutrient-dense, particularly in polyphenolic compounds. Previous research demonstrates benefits of whole grape and grape skin, seed, and polyphenol intake on glucose homeostasis along with other health benefits. We tested the hypothesis that intake of 4 servings per day of table grape would remediate metabolic complications in C57BL/6 J (C57) male mice fed a high-fat diet with added cholesterol and fructose diet modeling an obesogenic and diabetogenic western-style diet. Methods Groups of mice (n = 12) were provided either low-fat plus placebo diet (LF, 10% kcal fat), high-fat plus placebo (HF, 45% kcal fat), or HF plus grape powder (HF + G), for 8 weeks. Grape powder was provided at ∼10% of total energy of diet. C57 mice were provided experimental diets ad libitum. Body weights, food intake, and glucose tolerance were determined. Postmortem, inflammatory markers, cecal microbiome, and the relative concentrations of hepatic metabolites were determined. Results Fasting blood glucose was reduced in the HF + G group compared to HF-fed mice. The glucose tolerance test demonstrated that the Area Under the Curve (AUC) was also reduced. Further, a significant decrease in circulating levels of insulin were observed with HF + G supplementation. The cecal microbiome from HF + G fed mice overlapped with both the HF and LF controls, but also had characteristic shifts that were unique to grape powder consumption. Metabolomic analysis indicated grape consumption impacted inflammation and β-oxidation biomarkers indicating some remediation of hepatic pathologies associated with HF food consumption. The most significantly different hepatic metabolites included grape-derived S-methymethionine and trigonelline, while other murine hepatic metabolites significantly regulated by diet included myo-inositol and 15-HETE. Conclusions Table grape supplementation with a HF western-style diet significantly improved fasting blood glucose, circulating insulin concentrations, and HOMA-IR in C576J/Bl male mice. demonstrating an anti-diabetic effect of grape powder. At modest level of supplementation equivalent to 4 servings/day, grape powder also improved microbiome composition and changed relative levels of specific hepatic metabolites. Up-regulation of 15-HETE by diet suggests grape powder consumption may enhance PPARγ-directed gene expression, consistent with increases in glucose sensitivity observed in this study. Funding Sources California Table Grape Commission.