We developed a disposable electrode for the electroretinogram made of anomalous polyvinyl alcohol gel. This new hydrogel is a rubber-like elastic with water content 80-90 wt%, but insoluble in water. The elastic electrode plate is approximately 0.3-1.0 mm thick, sterile, flexible, and easily cut to the desired shape. Its low cost permits it to be discarded after use.

No abstract

The early effects of sodium iodate (NaIO3) on the ERG c- and b-waves were studied in rabbits. When 30 mg NaIO3/kg body weight was administered intravenously, the c-wave amplitude decreased and was replaced by a potential of opposite polarity. However, the administration of 20 mg/kg of NaIO3 enhanced the c-wave remarkably. Twenty min after administration, the mean c-wave amplitude significantly increased (P less than 0.001) to 211 +/- 6.2% of the pre-administration amplitude, whereas that of the control was 108 +/- 13.0%. The b-wave amplitude showed no significant changes. When 10 mg/kg of NaIO3 was administered, the enhancement of the c-wave was not remarkable. These effects were similar in both pigmented and albino rabbits. A lower blood concentration of NaIO3 applied to the basal side of the retinal pigment epithelium (RPE) may initially affect only the basal membrane of the RPE resulting in the decrease in the basal membrane resistance (Rba). This would explain the transient increase in the c-wave amplitude with 20 mg NaIO3/kg.

We examined some of the problems arising when c-waves are employed in pharmacological experiments with pigmented rabbits. The cyclic variations of the c-wave are smaller in rabbit than in other species. Under our experimental conditions the c-wave amplitudes in some rabbits reached an equilibrium under intermittent stimuli, while in others they increased with time and did not reach a constant level within 3 h. The c-wave amplitude varied with the standing potential during dark adaptation. However interindividual variations made it difficult to standardize the c-waves by this change. Since the c-wave amplitudes of the right and left eyes were similar, the contralateral eye should be used as a control when drugs are administered topically. Interindividual variations of the c-wave amplitude were smaller under dim light. This method is useful in chronic pharmacological experiments when drugs are administered systemically.

The large inter- and intra-individual variations in ERG c-waves have prevented the application of the component in pharmacological experiments on the retinal pigment epithelial function. We examined some of the problems involved in employing the c-wave in pharmacological experiments using rabbit retinas. 1) Under our experimental conditions, the c-wave amplitudes in some rabbits reached equilibrium under intermittent stimuli, while in others they increased with time and did not reach a constant level in 3 h. 2) The c-wave amplitudes of the right and left eyes were similar, indicating that the contralateral eye can be used as a control during local administration of drugs. 3) The c-wave amplitude under 50 lux light had smaller inter-individual variations than that in the dark. This may be a useful indicator of the retinal pigment epithelium function when drugs are administered systemically.