The current investigation was performed with the objective of finding the scientific reason for health benefit of Occimum sanctum, Phyllanthus niruri and Cadaba fruticosa, simultaneously evaluating the antioxidant activity of ethanol, methanol and aqueous extracts. The potent antioxidant, antimicrobial and anti-inflammatory leaf extracts can be used for producing a novel ointment formulation, functional food or neutraceutical, in combination. The qualitative analysis of antioxidant activity was performed by reducing power assay and DPPH assay and in vitro anti-inflammatory test was performed by the HRBC membrane stabilization method. Further, the bioactive compounds present in the alcoholic leaf extracts were detected by GC-MS analysis and their applications were noted. Comparative studies on antimicrobial and anti-inflammatory activity of the 3 samples were determined using respective standards. Cadaba fruticosa is an under-emphasized herb, whose potential in the medical world is yet to be explored in details. Hence, Cadaba fruticosa has been chosen as one of the herbal plants for comparative study with Ocimum sp. and Phyllanthus sp. Ethanolic extract of showed significant antimicrobial activity against the tested bacterial strains. Methanolic extract of Ocimum sanctum at 200 μg mLG 1 depicted highest scavenging activity, 81.1%. The results also indicate that Cadaba fruticosa possesses significant free radical scavenging and anti-inflammatory activities in vitro. GC-MS analysis of the herbs revealed the presence of several bioactive compounds.