Detection of fluorescence particles is a key method of flow cytometry. We evaluate the performance of a design for a microfluidic fluorescence particle detection device. Due to the planar design with low layer thicknesses, we avoid optical components such as lenses or dichroic mirrors and substitute them with a shadow mask and colored film filters. A commercially available LED is used as the light source and a PIN-photodiode as detector. This design approach reduces component cost and power consumption and enables supplying the device with power from a standard USB port. From evaluation of this design, we obtain a maximum particle detection frequency of up to 600 particles per second at a sensitivity of better than 4.7 × 10(5) MESF (molecules of equivalent soluble fluorochrome) measured with particles for FITC sensitivity calibration. Lowering the flow rate increases the instrument sensitivity by an order of magnitude enabling the detection of particles with 4.5 × 10(4) MESF.
We investigated the optoelectronic properties of solution processed organic light emitting diodes (OLEDs) as a function of their active layer thickness. By using a horizontal dipping technique and by accelerating the coating bar during wet film deposition, we fabricated OLED arrays with different emission layer thicknesses but identical process records in a single process step. The comparison of the optoelectronic device parameters allows for conclusions on injection limitation, the optimization of the layer thickness, and, in conjunction with optical simulations of the weak cavity effect, to promote a deeper understanding of the emission profile. To show the universality of this method, we investigated purely polymeric emitters, blends of polymers and small molecules as well as all-small molecule material systems.
We examine the impact of various parameters on the transient current density characteristics of organic solar cells and photodetectors by means of numerical simulations. Our self-consistent numerical model treats the dynamics of generated electrons and holes in the framework of a drift-diffusion model. As input parameter for the electric model, the intensity distribution of the incident light is calculated with a transfer-matrix method accounting for interference effects. The results are compared to experimental results. With our approach, we are able to distinguish the influence of different physical effects as they become dominant at different current densities or at different time regimes. This enables us to estimate the electron and hole mobilities separately by fitting the experimental results. Furthermore, space charge effects are identified as being highly important for the transient response of photodetectors.
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