AbstractParfume is one of the cosmetic product and its use is consistent. High prices of cosmetic product cause dependence on imported product. Innovative research is needed to reduce dependence on imported product. Melon is one of the potential horticultural crops to be developed in plant breeding. Gama Melon Parfum cultivars has strong of fragnant so its potentially to be used as parfume material.

-

Abstract. Maryanto SD, Wibowo WA, Daryono BS. 2021. Phenotypic characters and identification CYPs (Cyclophilin) gene in Cucumis melo L. cv. Gama Melon Parfum. Biodiversitas 22: 3007-3014. Cucumis melo L. cv. Gama Melon Parfum is a new cultivar with a very strong fragrance as its main character. As a new cultivar with unique characters, it is necessary to characterize phenotype and molecular related to the fragrant aroma. The research aimed to study the phenotypic of the fruit of C. melo cv. Gama Melon Parfum (F3) and to identify the CYPs gene as one of the genes that act in encoding volatile compounds. Analysis of qualitative characters was based on International Plant Genetic Resources Institute (IPGRI) and Plant Variety Protection (PVP), phenotypic characters in melons observed by abiding the Rules for Registration of Varieties from the Indonesian Minister of Agriculture Decree No. 700/Kpts/OT.320/D/12/2011, while analysis of quantitative characters was using the ANOVA methods and software PKBT-STAT-2. The methods of molecular characterization included RNA isolation, cDNA synthesis used Reverse Transcriptase-PCR, amplification of DNA target used PCR, visualization of DNA target used electrophoresis, and DNA sequencing. Analysis of the in silico approach was carried out on the CuGenDB Melon database. Analysis of protein sequences and classification was obtained from InterPro. Phylogeny analysis using MEGA-X Software. The results were 18 qualitative characters and 11 quantitative characters were stable and uniform, whereas the molecular characterization of the genes was predicted Cyclophilin with peptidylprolyl isomerase (PPIase) activity and located in chromosome 1 (17059021-17058899).

As many as 50 million people worldwide are currently experiencing a deficiency of vitamin A and about 250000-500000 malnourished children in developing countries become blind each year from vitamin A deficiency, with the highest prevalence in Southeast Asia and Africa which will result in visual impairment, and increased child mortality and pregnant women. Melon can be one of the solutions for higher productivity and fruit may contain carotene (pro-vitamin A) with varying levels. In the years 2009-2011 Genetics laboratory, faculty of Biology UGM has produced a new melon cultivar called Melodi Gama 1 (MG 1). MG 1 phenotype has a rounded character with rather thick rind and hard, green and fleshy with a smooth orange flesh color. The purpose of this research was to study and analyze the nutritional content of MG 1 compared to commercial cultivars from multilocation and multiseason cultivation. The methods were used vitamin A quantitative analysis by extraction and spectrophotometer, whereas vitamin C in quantitative analysis using the iodine titration method. The results obtained in the analysis of total carotene in the dry season for the MG 1 has the highest value compared with 4 other commercial cultivars, it was 706.61 μg/100gr for cultivation in Yogyakarta and 292.16 μg/100gr for cultivation in Magetan. In the rainy season total carotene MG 1 was 59.88 μg/100gr for cultivation in Yogyakarta, and 20.76 μg/100gr for cultivation in Magetan. The results of the quantitative analysis of vitamin C MG 1 in the dry season was 7.50 mg/100gr for cultivation in Yogyakarta and 16.50 mg/100gr for cultivation in Magetan, while the rainy season was 8.55 mg/100gr for cultivation in Yogyakarta and 6.75 mg/100gr for cultivation in Magetan. Based on these results it can be concluded that the content of Vitamin A and C from Melodi Gama 1 cultivar is higher in the dry season than in the rainy season.