There are considerable differences between the EU-15 countries and Central and Eastern European (CEE) countries in the application rate of phosphorus (P) fertilizer and manure as well as in the climate and erosion patterns, but the eutrophication of surface waters is a common problem. The availability of information on the contribution of the CEE countries to European surveys on eutrophication is not adequate, because the amount of data available from these countries is small. There is thus a need to compile more comprehensive data sets for better assessment. Recent changes in agricultural practices, and their impact on the trophic status of surface waters in CEE countries, have been evaluated using a few selected pressure and state indicators (phosphorus balance, phosphorus status and erosion of agricultural land, P loss risk index, chlorophyll a, total and orthophosphate content of water) by compiling and analysing data from the literature. In the CEE countries, small annual P balances ()7 to +6 kg P ha )1 ) and declining levels of soil available P (proportion of soils adequately supplied with P dropped to between 10 and 50% of total area) may lead to low yields and economic difficulties. Only 11% of the variation in total phosphorus (TP) in rivers studied could be attributed to the calculated P loss risk index, and thus to the impact of differences in the agricultural diffuse source P load. The generation of more consistent data sets should be pursued for the whole of Europe in order to derive more powerful indicators. A refined P index could be one of the best indicators of eutrophication. Recent changes in the trophic level of surface waters could be attributed to a large extent to the change in point source P loads, i.e. to the diminishing amount of P load from municipal and industrial wastewater. The short-term improvement of water quality in the CEE countries will require further improvements in wastewater treatment, but long-term improvement will only be achieved by reducing diffuse P losses from agriculture, possibly involving changes in the land use pattern and in farming practices.

Base temperature 8 °C Conservative Start temperature for plant growth 45 Sum of GDD Calibrated Specific leaf area of new leaves 0.004 ha leaf kg-1 dry matter Calibrated Potential CO2 assimilation rate of a unit leaf area for light saturation 58 kg CO2 ha-1 leaf h-1 Calibrated

No abstract

In order to characterize the response of selected grasses to water stress, relative water content (RWC) in leaves and quantum efficiency of photosystem 2 (F v /F m ) were measured in Phleum pratense L., P. bertolonii DC. and P. phleoides H. Karst. during 6 d of water stress. The results indicated differential responses to water stress among the three Phleum species with higher water deficit sensitivity of P. pratense and P. bertolonii than that of P. phleoides. The cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique was applied to identify differentially expressed genes responding to water stress in P. pratense. Cloned and sequenced differentially expressed fragments (DEFs) were used for primer design in order to identify orthologous genes in Lolium perenne L. Twelve genes orthologous to P. pratense DEFs were mapped in the L. perenne mapping population VrnA based on a high resolution melting curve analysis (HRM). This study provides genomic information about 29 differentially expressed genes after water stress in P. pratense and reports on the identification and mapping of twelve orthologs in L. perenne.

Climate change, new varieties, better technological abilities, and increased demand for local resources provide significant reasons to introduce soybeans in northern regions, above the typical soybean distribution area in Europe. This research examined the effects of two delayed sowing times, wide 25 cm and 50 cm row spacings, seed inoculation with Bradyrhizobium japonicum, and the interaction of all these factors on soybean development and productivity in an organic farming system. Length of soybean vegetation varied from 142 to 161 days at latitude 55° N. Yield varied from 673 to 3154 kg ha−1 in response to management factors. In the dry 2015 year, the combination of later sowing dates and wide 50-cm row spacing significantly (p < 0.01) increased the number of pods per plant by 28%, aboveground dry biomass by two times, and seed yield by 36% plant−1. In the wet 2016, yield components reached their highest values of 16.8 g dry biomass, 19.9 pods plant−1 and 7.9 g seeds plant−1 when inoculated soybeans were sown earlier, with 50-cm row spacings. Protein content significantly varied from 27.4 to 35.3%, and fat content 17.4–21.5%. This study suggests that regular soybean development could be maintained in organically managed locations above the present northern soybean distributional region, but its development, productivity, and production quality significantly depends on management practices.