Background: Cryoprecipitate is used without knowing the concentration of coagulation factors it contains which might cause circulatory overload with no any improvement if several bags are transfused with low factor levels. Objective: The objective was to assess the changes in coagulation factors in cryoprecipitate plasma during storage at -18°C for 5 weeks at Kisii Teaching and Referral Hospital. Methods: The study involved time series analysis design involving analysis of cryoprecipitate during storage at -18°C for 5 weeks. Blood collected from the Kisii satellite blood transfusion center was received at Hematology laboratory where factor assays were performed on Erba Mannheim ECL 105 semi-automated coagulation analyzer. Thawing for subsequent coagulation factor analysis and serial testing was done using Stericox Plasma Thawing Bath at 37°C, for 45 mins. Data were entered into Excel and analyzed by SPSS version 25. Results: The mean rank for cryoprecipitate from week one to week five was in a decreasing trend with 3.00, 1.99 and 1.01 respectively. This confirms a steady statistical significant difference in mean ranks for the time period. Conclusion: There was a significant reduction in coagulation factors in cryoprecipitate plasma during storage at -18°C for 5 weeks at Kisii Teaching and Referral Hospital.