Isothermal molecular techniques offer an alternative to single taxa monitoring using environmental DNA (eDNA). Methodologies such as Loop-Mediated Amplification (LAMP) (Notomi et al., 2000) and Recombinase Polymerase Amplification (RPA) (Piepenburg et al., 2006) have been readily adopted for point-of-care diagnostics (Craw & Balachandran, 2012;Oliveira et al., 2021), but use in environmental monitoring remains limited. Despite this, previous studies have shown successful detection of, for example, Dreissena sp. in the Great Lakes using LAMP (Williams et al., 2017) and harmful algae using RPA (Toldrà et al., 2019). Moreover, such isothermal amplification methods have the potential to be coupled to fluorescencebased detection such as through incorporation of an exo-probe (TwistAmp® exo RT kit) (Li et al., 2018) for real-time RPA or through coupling with a secondary technique such as CRISPR-Cas as reviewed in Kaminski et al. (2021).Both LAMP and RPA have been coupled with CRISPR-Cas technology to increase detection specificity (Broughton et al., 2020;