The steadily increasing demand for energy and concerns about climate change have prompted countries to promote the use of renewable energy sources, including lignocellulosic biomass. In this context, this work aims to assess the biomass production for energy purposes in crops with short rotation, as well as its effect on soil properties. Deciduous tree species were used, mainly Siberian elm (Ulmus pumila L.), black locust (Robinia pseudoacacia L.) and a hybrid poplar clone (Populus × euroamericana (Dode) Guinier, clone ‘AF2′). Four field trials were implemented, under two different types of Mediterranean climate, where highly productive taxa were tested, in addition to the mixed planting of a nitrogen-fixing species with a non-fixing one. Short-rotation coppices (SRCs) of these taxa yield about 12–14 t ha−1 year−1 of high-quality dry woody biomass, when fertilizers and irrigation water are supplied; generate 205–237 GJ ha−1 year−1 net and earnings of about EUR 1.5 per EUR 1 invested; and sequester into the soil 0.36–0.83 t ha−1 year−1 of C and 57 kg ha−1 year−1 of N. Therefore, these species raised as SRCs could improve degraded soils if the crop is properly managed, resulting in favorable economic, energy and CO2 emission balances. The use of mixed plantations can bring economic and environmental gains, and the biomass transformation into high-quality chips or pellets gives it added value.