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The human impact upon the subterranean microbiomes is not only a peril to the cave environment but might also affect future visitors. We focused on the changes that humans induced on the surfaces they came in direct or indirect contact with inside two intensely visited Romanian show caves, by means of commercially available microbial rapid test kits and molecular identification. Overall culturable bacteria abundance in the caves maintained high levels year-round while Enterobacteriaceae, coliform bacteria and Escherichiacoli levels peaked during the touristic season, reaching levels that could pose a threat to the health of the visitors. Culturable fungi abundance usually peaked in the spring, remained at a high level in the summer and started to slowly decrease towards the winter months. Differences were observed between the direct and indirect exposed surfaces, as the later had lower overall levels of bacteria and fungi, with increased Enterobacteriaceae loads. Most of the taxa identified are known biodeteriorants of subterranean surfaces and were previously associated with human altered caves. A Dothideomycete sp. previously unknown to the cave environments was detected. This was the first study to analyse the dynamics of the microbial communities of delicate subterranean surfaces in show caves through the use of commercially available test kits. We revealed that exposed surfaces in show caves, in direct or indirect contact with tourists, are host to high concentrations of cultivable microbes. The touristic activity was shown to influence the abundance and dynamics of the microbial communities inhabiting surfaces of show caves.

Proper health knowledge and adequate motivation for health activities are key factors that influence an individual to adopt a healthy behavior. Health promotion positively influences progressive behaviors that seek to advance health potential, to continuously improve one’s lifestyle. There are many health promotion indications constantly encouraging people to eat healthier food. Based on the successful experience of a non-profit organization promoting a healthier vegetarian diet, this research identifies the operating factors that lead to the success of health promotion. The formulation and implementation of the health promotion strategy must be combined with the key success factors in order to accomplish the objectives. This study assessed seven factors, evaluated using the proposed method. The proposed Decision Making Trial and Evaluation Laboratory (DEMATEL) method constructs the cause and effect model of health promotion, and places forward suggestions and strategies for improvement based on the evaluation of the results. This research compared the original DEMATEL with a Modified DEMATEL (M-DEMATEL) to identify the success factors of health promotion. According to the results of both methods, “leadership”, “communication channel” and “budget” are the most important and influential factors when promoting healthy diets. The results have shown the connection and the difference between the two methods. The main purpose of this research is not to determine which method is the best method, instead, to derive the combined effect of both methods.