The causative viral agent was purified from diseased shrimp Penaeus japonicus with white spot syndrome (WSBV). Several hundred clones were obtained from libraries of the purified viral genomic DNA. According to the results of nucleotide sequence analysis, none of the WSBV clones showed considerable sequence homology with those of other known viruses, indicating that WSBV is a new virus causing a serious disease in shrimp. Based on the sequence data of WSBV genomic DNA, a pair of polymerase chain reaction (PCR) primers was designed. After 30 cycles of PCR amplification of viral genomic DNA extracted from WSBV, a single product of the expected size was detected. Southern blot hybridization confirmed that the amplified product was specific to the DNA of WSBV. The PCR system was able to detect 1 pg of WSBV DNA after 30 cycles, and efficiently amplify the target region of WSBV gene in the total nucleic acids extracted either from the diseased shrimp or hatchery shrimp with no signs of viral infection.

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This research discusses about the phenomena of blended code in Arabic conversation of students of Madinah Modern Islamic Boarding School. This phenomena happen because a majority of students in saying Arabic are still influenced by local language and less balance in mastering two languages. So that, often happening deviation and appearing language case names mingled code. Related with the gotten blended code data, the researcher uses blended code theory filed by Muysken for analyzing the data. There are three blended cose by Muysken are insertion, alternation, and congruent lexicalization. The method which is used in this research are observation and interview method. First: observation method, this method has base technique. It is a skillful involved free observed technique note, and record. Second: interview method, at interview method applied by inducement technique, this technique is used as base technique and record technique as continuing technique. The objective of this research is to identify mingled code in Arabic conversation of students of Madinah Boarding School, local language elements in Arabic.

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