Nowadays simple usability is one of the key tasks of web portals, especially if these are main information portals of the government. The aim of this paper is to evaluate the usability of a web portal eAGRI (eagri.cz) in terms of unregistered users. This means in terms of general public in particular. The main purpose of the testing is to highlight the issues that users may encounter on this portal and determine the level of portal usability. Our usability testing was focused on a public part of the portal. The main idea of eAGRI portal is to create a central access point to information resources of the Ministry of Agriculture of the Czech Republic and its subordinate organizations. Qualitative research methods were applied. Specifically, we used Heuristic evaluation as a usability inspection method and three methods of usability testing: 5 second test, 30 second test and Formalized think-aloud test. KeywordsUsability analysis, eAGRI portal, heuristic evaluation, user testing, 5 second test, 30 second test.