This paper analyses whether hosting the most prestigious European cultural event, the European Capital of Culture, has an impact on regional economic development and the life satisfaction of the local population. We show that European Capitals are hosted in regions with above-average GDP per capita, but do not causally affect the economic development in a significant way. Surprisingly, using difference-in-difference estimations, a negative effect on the wellbeing of the regional population is found during the event. Since no effect is found before the event, reverse causality and positive anticipation can be ruled out. The negative effect during the event might result from dissatisfaction with the high levels of public expenditure, transport disruptions, general overcrowding or an increase in housing prices.

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Hydroxylation of polyaromatic compounds through cytochromes P450 (CYPs) is known to result in potentially estrogenic transformation products. Recently, there has been an increasing awareness of the importance of alternative pathways such as aldehyde oxidases (AOX) or Nmethyltransferases (NMT) in bioactivation of small molecules, particularly N-heterocycles. Therefore, this study investigated the biotransformation and activity of methylated quinolines, a class of environmentally relevant N-heterocycles that are no native ligands of the estrogen receptor (ER), in the estrogenresponsive cell line ERα CALUX. We found that this widely used cell line overexpresses AOXs and NMTs while having low expression of CYP enzymes. Exposure of ERα CALUX cells to quinolines resulted in estrogenic effects, which could be mitigated using an inhibitor of AOX/NMTs. No such mitigation occurred after coexposure to a CYP1A inhibitor. A number of N-methylated but no hydroxylated transformation products were detected using liquid chromatography−mass spectrometry, which indicated that biotransformations to estrogenic metabolites were likely catalyzed by NMTs. Compared to the natural ER ligand 17β-estradiol, the products formed during the metabolization of quinolines were weak to moderate agonists of the human ERα. Our findings have potential implications for the risk assessment of these compounds and indicate that care must be taken when using in vitro estrogenicity assays, for example, ERα CALUX, for the characterization of N-heterocycles or environmental samples that may contain them.

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ZusammenfassungWarum es heute vielleicht wichtiger denn je ist, einen Sexratgeber für Frauen zu schreiben und zu lesen, erklärt der vorliegende Debattenbeitrag. Als Verfasserin eines feministischen Sexualaufklärungsbuchs beobachtet die Autorin die sexpositiven Internet-Bubbles und kennt auch die kritischen Reaktionen dazu. Insgesamt sieht sie die Sache mit der weiblichen Lust momentan verhalten optimistisch.