Objectives: This study aimed to evaluate Osteopathic Manipulative Treatment (OMT) effects on biliary dyskinesia using paired ultrasound with elliptical vesicle measurement. Methods: Sixty-four individuals with biliary dyskinesia were enrolled in this study (14 men and 50 women) and divided into two groups, the treatment group that received OMT in the global and gallbladder area (visceral manipulation) and the control group that received only global OMT. Two ultrasound exams, fasting, and 50 minutes after breakfast were performed to exclude lithiasis diagnosis and measure volume (Dodds elliptical method) and ejection fraction and percentage of gallbladder before and after 30 days of treatment. Results: The ejection and filling gallbladder percentage increased significantly after 30 days in the OMT group. Conclusion: OMT may be a noninvasive strategy for the treatment of biliary dyskinesia.