The metabolites produced when Monocillium nordinii (Bourchier) W. Gams, a destructive mycoparasite of pine stem rusts, is grown in liquid culture have been separated and identified. The metabolites include the known compound monorden (1) and five new substances, monocillin I (2), monocillin II (4), monocillin III (3), monocillin IV (5), and monocillin V (6). Structural assignments and chemical correlations of the five new compounds are reported and the absolute configuration of monorden is assigned. The antifungal spectra of the three major metabolites are reported. Monorden and monocillin I show pronounced activity against a wide variety of fungi, including Ceratocystis ulmi, the cause of Dutch elm disease. Extraction of the mycelium yielded averufin (13), along with a pigment C18H12O6, as yet unidentified.
Seven biospecific adsorbents containing immobilized androgens were synthesized: dihydro‐testosterone‐17β‐succinyl agarose, and both the unsubstituted and the 17β‐acetyl derivatives of dihydrotestosterone‐7α‐undecanoyl agarose, testosterone‐7α‐undecanoyl agarose, and 19‐nortestosterone‐7α‐undecanoyl agarose. The retention capacities for nuclear androgen receptor were generally between 40‐80% with little variation in reproducibility; the amount of binding was greatest with dihydrotestosterone‐17β‐succinyl agarose and dihydrotestoster‐one‐17β‐acetoxy‐7α‐undecanoyl agarose. Rapid flow rates were obtained with all gels, and no tendency for decomposition was observed over a period of 1 year. Factors that affected retention included the concentration of immobilized androgen, length of the linker arm, occupation of receptor sites, interval of contact with the gel, and temperature of incubation. Chemical dissociation of androgens from androgen receptor complexes with 0.2 mM mersalyl increased the retention of receptor by dihydrotestosterone‐17β‐succinyl agarose. Two elutants showed promise for the dissociation of gel‐bound receptor: 1) 0.2 mM mersalyl in the presence of 1.5 mg/ml of ovalbumin; 2) 10% (v/v) dimethylformamide:water containing 30 μM [1,2‐3H] dihydrotestosterone and 0.5 M sodium thiocyanate.
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