>The hydroxypropyl methylcellulose (HPMC) based transdermal patches of aceclofenac were prepared. On the basis of in-vitro potential, the formulation was additionally chosen and assessed for their in-vivo anti-arthritic activity and acute skin irritation tests using the primary skin irritation (draize) test and freund’s complete adjuvant (FCA) caused arthritis technique. Furthermore, stability studies were conducted in compliance with ICH standards. The studied compositions had no irritant potential, and no edema production was ever noticed. All of the formulations had a zero irritation score (primary skin irritation index), indicating their safety and acceptability for transdermal administration. The stability investigations confirmed the produced patch’s stability, and the in-vivo analysis showed a considerable decrease in the volume of irritated paws compared to the marketed formulation. The created transdermal patches may serve as a platform for the delivery of medication for the treatment of arthritis