Background: Currently assessment of autistic behavior is done based on learning disabilities, personal observation of behavioral patterns and standard autistic scales. Electrophotonic imaging (EPI) instrument is used to assess health status based on bio-energy field of various organ and organ system of human body. And can be useful to determine the early diagnosis of autistic symptoms and degree of improvement for any therapeutic intervention given to these autistic children on a regular basis. This study aimed to investigate the differences of EPI parameters of autistic children and healthy children of the same age group.Methods: This study was carried out by taking the EPI images of 33 autistic and 36 healthy children of age group 4 to 14 years from an autistic center and nearby school in Bangalore. The statistical analysis on acquired data were done using IBM SPSS Version 20.0.Results: The variables activation coefficient, integral area, sacrum, hypothalamus, thyroid gland, pancreas and coronary vessels showed a significant statistical difference in their mean value for autistic and healthy children (p<0.05).Conclusions: The EPI parameters for autistic and healthy children open up the possibility of using EPI based instrument for early diagnosis. Deeper analysis of the differing parameters gave us more insight into the type of intervention to be selected for improving the health of autistic children.