Grazing rate of copepods was evaluated in the oceanic province of the Colombian Pacific during September 2006. Gut pigment content was estimated by spectophotometry considering three size fraction: small (300-500 µm), medium (500-1000 µm) and big (> 1000 µm). In order to estimate the ingestion rate, an experimental gut evacuation rate of 0.702 h -1 was used. Feeding impact was calculated like a proportion of phytoplankton standing stock, projecting the total copepod consumption by size in relation to chlorophyll-a concentration in each station. Small copepods were most abundant than medium or big copepods (Kruskal-Wallis H=21.83; p=0.01). Although the ingestion rate of big copepods was higher than medium or small copepods (Kruskal-Wallis H=6.48; p= 0.04), no significant differences in the consumption rate were detected among sizes (Kruskal-Wallis H=3.07; p=0.21). The chlorophyll-a integrate (0-50 m) in the study zone was between 4.4 and 9.5 mg Clo-a m -2 , being estimated the grazing impact of the big copepod in 2.57 ± 0.80 %, medium 3.57 ± 1.89 % and small 12.06 ± 5.99%.Keywords: grazing, ingestion rate, chlorophyll, copepods, Pacific Ocean, Colombia. Impacto alimentario de los copépodos calanoideos en elOcéano Pacífico colombiano ResumenSe determinó el impacto alimentario de los copépodos en nueve estaciones localizadas en la Cuenca Pacífica Colombiana durante septiembre de 2006. El contenido de pigmentos entéricos, fue medido espectrofotométricamente en tres fracciones de tamaño: pequeño (300-500 µm), mediano (500-1000 µm) y grande (> 1000 µm); y se estimó la tasa de consumo considerando una tasa de evacuación de 0.702 h -1 que fue establecida previamente de manera experimental. Considerando la abundancia de cada fracción y la concentración de clorofila-a en cada estación, se cuantificó el impacto alimentario como un porcentaje de la biomasa fitoplanctónica disponible. Los copépodos de menor tamaño fueron significativamente más abundantes en la zona de estudio (Kruskal-Wallis H=21.83; p=0.01). Aunque la tasa de ingestión de los copépodos grandes fue significativamente mayor (Kruskal-Wallis H=6.48; p= 0.04), no se detectaron diferencias significativas entre las tasas de consumo de las tres fracciones de tamaño evaluadas (Kruskal-Wallis H=3.07; p=0.21). La clorofila-a integrada (0 -50 m) en la zona de estudio estuvo entre 4.4 a 9.5 mg Clo-a m -2 , siendo el impacto alimentario promedio de los copépodos grandes de 2.57 ± 0.80 %, medianos 3.57 ± 1.89 % y pequeños 12.06 ± 5.99%.
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