This study aimed to investigate the influence of emotional intelligence on employee psychological well-being with altruism as a moderator. This study was a cross-sectional study using quantitative methods, through the distribution of questionnaires. A total of 194 respondents were selected among employees of a government agency in Malaysia. The USM Emotional Quotient Inventory (USMEQ-i) was used in this research to measure emotional intelligence, The Psychological General Well-Being Index (PGWBI) was used to measure psychological wellbeing and Adapted Self-Report Altruism Scale was used to measure altruism. The results showed that emotional intelligence has an influence on psychological well-being of employees positively and significantly. However, the results found that altruism did not moderate the relationship between emotional intelligence with psychological wellbeing of employees.