Micropropagation is a vegetative propagation conducted under controlled and aseptic conditions in the microenvironment of the culture vessel, which have the all growth requirements of a plant in the natural conditions. Recently different techniques of propagation have been developed which could facilitate large scale production of plants and for the improvement of the species. An overview on the in vitro propagation via meristem culture, callus culture and protoplast culture etc. are presented here. Today micropropagation techniques are applied in order to produce large numbers of new high-quality plants in a relatively short time and space, in low cost and can also be preserved.

Aim:The aim of this study is to detect in vitro adherence of Enterococcus faecalis to the infected dentinal tubules of human extracted teeth using confocal laser scanning microscope.Subjects and Methods:Roots from human premolar teeth (n = 40) were infected with E. faecalis strain the American Type Culture Collection 29212 in brain heart infusion for 21 days. After the experimental period, specimens were divided into two groups, Group A (n = 20), Group B (n = 20), and Group A specimens were stained with fluorescein diacetate dye for the detection of viability and adherence Group B were stained with acridine orange dye for detection of metabolic activity and adherence. Samples were washed, thoroughly sectioned and examined by confocal laser scanning microscopy. Computer-assisted determinants of fluorescence, bacterial viability, metabolic activity, and adherence were compared statistically.Results:E. faecalis was able to invade the dentinal tubules to a depth of 1–400 μm and adhere to 1–200 μm depth. Adherence (90%) was significantly higher in 1–100 μm using fluorescein diacetate and acridine orange dye.Conclusion:Adherence of E. faecalis as evaluated by confocal laser scanning microscope was highest at the depth of 1–100 μm which may have an impact on the shaping and cleaning procedures on the root canal.

Anesthesia simply means, without sensation induced for medical purposes include analgesia, paralysis, amnesia or unconsciousness. A of anesthetic drugs is referred to as being anesthet and Local anesthesia. General anesthesia sensation Examples are halothane and ketamine etc whereas, Local nerve impulses from a specific part of the body Example has various applications in field of medical like hypnosis uses it also have many complications duri salivation, convulsions, nausea, Vomiting, Pneumonia and Persisting sedation.