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N and P double doped porous carbon derived from Sonchus arvensis L at different carbonization temperatures (700 °C, 800 °C and 900 °C) were prepared by a simple one-step activation pyrolysis for the simultaneous electrochemical detection of AA, DA and UA. Compared with SaL-700 and SaL-900, the Sal-800 show excellent electrochemical sensing ability. Therefore, further electrochemical sensing studies were carried out by using SaL-800. The linear range of AA was 200-6000 μM., the sensitivity was 0.06 μA·μM-1·cm-2, and the detection limit was 76 μM (S/N=3). The sensitivity of DA was 9.81 μA·μM-1·cm-2 (0.5-20 μM) and 39.69 μA·μM-1·cm-2 (20-90 μM), and the detection limit was 0.11 μM (S/N=3). The sensitivity of UA was 0.81 μA·μM-1·cm-2 (10-100 μM) and 4.05 μA·μM-1·cm-2 (100-900 μM), and the detection limit was 2.70 μM (S/N=3). In addition, satisfactory results have been obtained for the determination of AA, DA and UA in normal human serum, which provides a new research direction for the construction of electrochemical sensors in the future.

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