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Papanicolaou staining is recommended for routine cervical cytology and cervical cancer screening. Our study was conducted by adapting modied staining technique which involves replacement of use of descending grades of alcohol with one step of 80% isopropyl alcohol. Prosafe papanicolaou staining was intended to reduce the staining time and cost of staining without compromising the quality or cytodiagnosis of the smear.

Introduction: Pap stain is a universal stain which is used for cytological screening. It is a very reliable technique. There are certain limitations of routine PAP staining like using of ethanol as dehydrating agent which is costly, hazardous and color preservation is not long standing. Our aim Aim: was to design a method using a limited amount of ethyl alcohol and it had to be rapid and economical as standard Papanicolaou stain without compromising staining quality. Materials And Merhods: A total of 200 smears collected from the endometrium of 100 unxed total hysterectomy specimens, 2 smears from each specimen, received in Pathology department of S Nijalingappa Medical College, Bagalkot. Of these, 100 smears underwent standardization of the Hazard free Pap staining and their counterparts underwent routine Papanicolaou staining. Results: The two methods were compared in respect of staining quality, cytoplasmic, nuclear details, time, cost and its ecofriendly feature. Staining time was minimized to 4 minutes from 20 minutes.In Hazard free staining, the nuclear and cytoplasmic staining was optimal in 90 and 93 respectively. Conclusion: Hazard free Pap stain provides a suitable, excellent and rapid alternative for cytological screening with minimum cost, lesser time and being ecofriendly by avoiding ethanol.