The present study was conducted to know the effect of nutritional supplementation of neonatal pig on biochemical profile during pre-weaning period. A total of 72 number of Large White Yorkshire nursing piglets were utilized. Animals were randomly assigned into 4 equal groups; T1, T2, T3 & C (control). Weaning was done on day 28. The nutritional supplement containing bovine colostrum, egg yolk, probiotic, zinc oxide, chelated iron, dextrose powder etc. was fed orally twice daily with a gap of 6 hours up to 7 days to the piglet @ 2, 4, 6 ml/kg body wt./piglet of group T1, T2 and T3 respectively. The control group was not fed any nutritional supplementation. Blood samples of piglets were analysed for Glucose, Total Protein (TP), Albumin, Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), and Blood Urea Nitrogen (BUN) at 7 th , 14 th , & 28 th day of experiment. The mean±SE of biochemical parameters in pre-weaning piglets were in normal ranged in group T1, T2 and T3 and control group and significance difference were observed in glucose level on day 7 and albumin level on day 14 of preweaning. However, overall biochemical level at 28 th day was in normal range which indicates that supplementation of nutritional supplement during pre-weaning stage did not influence the biochemical parameters in pre-weaning pigs.

Background: Osteopontin (OPN) is an extracellular matrix protein with multifunctions and has been identified in the reproductive tract of both males and females. OPN from the seminal plasma (SP) has been closely associated with male fertility in several domestic animals. Different isoforms of OPN has been identified in the SP and sperm membrane in dogs but with unknown functions. The current study investigated the comparison of key canine sperm microscopic parameters and SP markers between dogs exhibiting different fertility potential.Methods: In this investigation during 2018-2019, 21 dogs coming for breeding soundness examination were selected and divided into fertile (Group I) and hypofertile (Group II). Evaluation included assessment of sperm microscopic characteristics and estimation of OPN, Malondialdehyde (MDA), Catalase (CAT), Glutathione peroxidase (GPx) and Superoxide dismutase (SOD) concentration in the SP.Result: The OPN level showed positive correlation with all the sperm evaluated characteristics (p less than 0.01). Linear regression analysis depicted a significant (p less than 0.001) influence of OPN on the evaluated sperm characteristics. These findings suggest that OPN in the SP along with the microscopic characteristics of the spermatozoa can be used as a potential fertility marker in discriminating fertile from the hypofertile ones.

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