Saccharopolyspora indica sp. nov., an actinomycete isolated from the rhizosphere of Callistemon citrinus (Curtis) Electron Microscope Facility, NIN, Tarnaka, Hyderabad-500007, Andhra Pradesh, IndiaA novel actinomycete strain, designated VRC122 T , was isolated from a Callistemon citrinus rhizosphere sample collected from New Delhi, India, and its taxonomic status was determined by using a polyphasic approach. Strain VRC122T was a Gram-stain-positive, aerobic, non-motile, non-acid-alcohol-fast strain. Phylogenetic analysis based on 16S rRNA gene sequences showed the strain was placed in a well-separated sub-branch within the genus Saccharopolyspora. The highest levels of 16S rRNA gene sequence similarity were found with Saccharopolyspora hirsuta subsp. kobensis JCM 9109 T (98.71 %), Saccharopolyspora antimicrobica I05-00074 T (98.69 %) and Saccharopolyspora jiangxiensis W12 T (98.66 %); 16S rRNA gene sequence similarities with type strains of all other species of the genus Saccharopolyspora were below 98 %. Chemosystematic studies revealed that it contained meso-diaminopimelic acid. Arabinose and galactose were the predominant whole-cell sugars. Diagnostic polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylcholine. MK-9(H 6 ) was the predominant menaquinone. C 14 : 0 , C 16 : 0 , iso-C 15 : 0, iso-C 16 : 0 , iso-C 17 : 0 , anteiso-C 15 : 0 , anteiso-C 17 : 0 , C 17 : 0 cyclo and summed feature 3 (C 16 : 1 v7c and/or C 16 : 1 v6c) were the major cellular fatty acids. The G+C content of the genomic DNA was 69.5 mol%. The results of DNA-DNA hybridization (30 %, 22 % and 25 %, respectively) with type strains of the above-mentioned species, in combination with differences in physiological and biochemical data supported that strain VRC122 T represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora indica sp. nov., is proposed. The type strain is VRC122 T (5KCTCThe genus Saccharopolyspora was first described by Lacey & Goodfellow (1975) and was assigned to the family Pseudonocardiaceae (Warwick et al., 1994). At the time of writing, the genus consists of 21 recognized species, namely Saccharopolyspora hirsuta (Lacey & Goodfellow, 1975) cavernae ' (Cheng et al., 2013), have been published but the names that are yet to be validly published. In the present study, we describe the taxonomic characterization of a novel species of the genus Saccharopolyspora. Strain VRC122T was isolated from a rhizosphere sample of Callistemon citrinus (Curtis) Skeels, from New Delhi, IndiaThe GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain VRC122T is JX411621. (GPS coordinates for the sampling site: 28 u 369 500 N 77 u 129 320 E), with the prospect that they might produce novel antimicrobial agents. Soil samples were collected in sterile tubes and brought to the laboratory of Osmania University, Hyderabad, India. Samples were dried in laminar flow under aseptic conditions. By using the serial dilution technique, samples were made up to 10 25 dilu...

A novel actinomycete strain, designated VRC07(T), was isolated from a Callistemon citrinus rhizosphere sample collected from Hyderabad, India. Its taxonomic status was determined by using polyphasic approach. It is a Gram-positive, aerobic, non-motile, weakly acid-fast strain. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain VRC07(T) is a member of the genus Nocardia. The highest levels of 16S rRNA gene sequence similarity was found between the strains Nocardia niwae W9241(T) (99.6 %), Nocardia amikacinitolerans W9988(T) (99.3 %) and Nocardia arthritidis IFM 10035(T) (98.9 %); similarity to other type strains of the genus Nocardia was below 98.7 %. The organism had chemical and morphological features consistent with its classification in the genus Nocardia such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall peptidoglycan. Arabinose and galactose as the diagnostic sugars. Diagnostic polar lipids were phosphatidylinositol, diphosphatidylglycerol, and phosphatidylglycerol. The predominant menaquinone was MK-8(H4, ω-cycl). The major fatty acids were C16:0, C18:0, C18:1 w9c, C18:0 10-methyl TBSA and sum in feature 3 (16:1 w7c/16:1 w6c). The G+C content of the genomic DNA was 68.5 mol%. The DNA-DNA relatedness data, together with phenotypic differences clearly distinguished the isolate from its closest relatives. On the basis of these phenotypic and genotypic data, the isolate represents a novel species, for which the name Nocardia bhagyanesis sp. nov., is proposed. The type strain is VRC07(T) (=KCTC 29209(T) = MTCC 11725(T) = ATCC BAA-2548).

Subsequent to the publication of the above paper it has been brought to our attention that the species epithet proposed for the taxon represented by strain VRC07 T is not grammatically correct as ''Nocardia bhagyanesis'' should have been proposed as Nocardia bhagyanarayanae. We propose here the corrected name for the taxon represented by strain VRC07 T. Description of Nocardia bhagyanarayanae sp. nov. Nocardia bhagyanarayanae sp. nov. (bha.gy.a.na.ra.ya'nae: N.L. fem. gen. n. bhagyanarayanae, of Bhagyanarayana, named in the honour of Prof. Bhagyanarayana Gaddam, a renowned Indian mycologist). The description of the species is as given for ''Nocardia bhagyanesis'' in Vaddavalli et al. (2014) Antonie van Leeuwenhoek 105:443-450. The type strain is strain VRC07 T (= KCTC 29209 T = MTCC 11725 T = ATCC BAA-2548 T).